Molecular Epidemiology of -Positive Polymyxin B-Resistant Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China.

, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing -positive in Shandong, China. We collected 668 non-duplicate ESBL-producing strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the 1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene , with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the gene into J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene . The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no IS insertion sequences were found on either side of the gene. Our study uncovered the molecular epidemiology of -carrying ESBL-producing in the region and suggested horizontal transmission mediated by plasmids as the main mode of transmission.