Chen Huan, Gu Tingting, Lv Longyang, Chen Xing, Lu Qifeng, Kotb Amer, Chen Wei
School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China.
School of Chips, XJTLU Entrepreneur College (Taicang), Xi'an Jiaotong-Liverpool University, Taicang, Suzhou 215400, China.
Nanomaterials (Basel). 2024 Aug 31;14(17):1430. doi: 10.3390/nano14171430.
A biocompatible, highly sensitive, and enzyme-free glucose electrochemical sensor was developed based on a copper-cysteamine (Cu-Cy)-modified electrode. The catalytically active biocompatible material Cu-Cy was immobilized on the electrode surface by the natural polymer chitosan (CTS). The electrochemical characterization and glucose response of the Cu-Cy/CTS/glassy carbon electrode (GCE) were investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and constant potential amperometry. The significant electrocatalytic activity of Cu-Cy to the oxidation of glucose in an alkaline environment was revealed. Several crucial parameters, including the number of scanning cycles for electrode activation, applied potential, and the contents of Cu-Cy and chitosan, were investigated to understand their impact on the sensor's response. The proposed sensing platform exhibited linear ranges of 2.7 μM to 1.3 mM and 1.3 mM to 7.7 mM for glucose detection, coupled with high sensitivity (588.28 and 124.42 μA·mM·cm), and commendable selectivity and stability. Moreover, a Cu-Cy/CTS-modified screen-printed electrode (SPE) was further developed for portable direct detection of glucose in real samples.
基于铜-半胱胺（Cu-Cy）修饰电极开发了一种具有生物相容性、高灵敏度且无酶的葡萄糖电化学传感器。具有催化活性的生物相容性材料Cu-Cy通过天然聚合物壳聚糖（CTS）固定在电极表面。采用电化学阻抗谱（EIS）、循环伏安法（CV）和恒电位安培法研究了Cu-Cy/CTS/玻碳电极（GCE）的电化学特性和葡萄糖响应。揭示了Cu-Cy在碱性环境中对葡萄糖氧化具有显著的电催化活性。研究了几个关键参数，包括电极活化的扫描循环次数、施加电位以及Cu-Cy和壳聚糖的含量，以了解它们对传感器响应的影响。所提出的传感平台在葡萄糖检测中表现出2.7 μM至1.3 mM和1.3 mM至7.7 mM的线性范围，同时具有高灵敏度（588.28和124.42 μA·mM·cm）以及良好的选择性和稳定性。此外，还进一步开发了一种Cu-Cy/CTS修饰的丝网印刷电极（SPE），用于实际样品中葡萄糖的便携式直接检测。
