Chen Lulu, Zhang Yue, Bu Yufen, Zhou Junhui, Man Yi, Wu Xinyuan, Yang Haobo, Lin Jinxing, Wang Xiaodong, Jing Yanping
State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China.
National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China.
J Exp Bot. 2024 Dec 4;75(22):6980-6997. doi: 10.1093/jxb/erae384.
Plant hormones are essential and structurally diverse molecules that regulate various aspects of plant growth, development, and stress responses. However, the precise analysis of plant hormones in complex biological samples poses a challenge due to their low concentrations, dynamic levels, and intricate spatial distribution. Moreover, the complexity and interconnectedness of hormone signaling networks make it difficult to simultaneously trace multiple hormone spatial distributions. In this review, we provide an overview of currently recognized small-molecule plant hormones, signal peptide hormones, and plant growth regulators, along with the analytical methods employed for their analysis. We delve into the latest advancements in mass spectrometry imaging and in situ fluorescence techniques, which enable the examination of the spatial distribution of plant hormones. The advantages and disadvantages of these imaging techniques are further discussed. Finally, we propose potential avenues in imaging techniques to further enhance our understanding of plant hormone biology.
植物激素是必不可少且结构多样的分子，它们调节植物生长、发育和应激反应的各个方面。然而，由于植物激素在复杂生物样品中的浓度低、水平动态变化且空间分布错综复杂，对其进行精确分析面临挑战。此外，激素信号网络的复杂性和相互关联性使得难以同时追踪多种激素的空间分布。在本综述中，我们概述了目前公认的小分子植物激素、信号肽激素和植物生长调节剂，以及用于分析它们的分析方法。我们深入探讨了质谱成像和原位荧光技术的最新进展，这些技术能够检测植物激素的空间分布。还进一步讨论了这些成像技术的优缺点。最后，我们提出了成像技术的潜在发展方向，以进一步增进我们对植物激素生物学的理解。
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