Yang Xiaopeng, Zhang Shiyi, Wang Haiyang, Lai Miao, Zhang Di, Ji Xiaoming
College of Tobacco Science, Henan Agricultural University, Zhengzhou 450046, China.
Institute of Agricultural Quality Standards and Testing Technology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
J Agric Food Chem. 2025 May 7;73(18):10701-10735. doi: 10.1021/acs.jafc.5c02335. Epub 2025 Apr 28.
Plants are constantly exposed to various stressors that can severely hinder their growth and threaten agricultural productivity. Recent advancements in plant imaging using fluorescent probes have opened new avenues for exploring the intricate processes involved in plant stress perception and signaling. This review represents the comprehensive effort to consolidate recent advances in fluorescent probe technologies, encompassing small-molecule probes, nanoprobes, and genetically encoded indicators, as revolutionary tools for deciphering stress-induced physiological dynamics. We present a comprehensive classification of fluorescent probes designed for detecting key biomolecules involved in plant stress responses, including reactive species, phytohormones, enzymes, and other signaling molecules. By critically evaluating their design principles, practical applications, and distinct advantages over conventional analytical methods, we aim to empower plant scientists in unraveling the spatiotemporal regulation of stress signaling networks. Finally, we propose strategic directions to overcome current technical bottlenecks and maximize the potential of fluorescence-based sensing in advancing sustainable agriculture.
植物不断受到各种应激源的影响，这些应激源会严重阻碍其生长并威胁农业生产力。利用荧光探针进行植物成像的最新进展为探索植物应激感知和信号传导所涉及的复杂过程开辟了新途径。本综述全面整合了荧光探针技术的最新进展，包括小分子探针、纳米探针和基因编码指示剂，这些都是用于解读应激诱导的生理动态的革命性工具。我们对用于检测参与植物应激反应的关键生物分子的荧光探针进行了全面分类，包括活性物质、植物激素、酶和其他信号分子。通过严格评估它们的设计原理、实际应用以及相对于传统分析方法的独特优势，我们旨在帮助植物科学家解开应激信号网络的时空调控机制。最后，我们提出了战略方向，以克服当前的技术瓶颈，并最大限度地发挥基于荧光传感在推进可持续农业方面的潜力。
