Recent Advances in Fluorescent Probe for Detecting Biorelevant Analytes during Stress in Plants.

Plants are constantly exposed to various stressors that can severely hinder their growth and threaten agricultural productivity. Recent advancements in plant imaging using fluorescent probes have opened new avenues for exploring the intricate processes involved in plant stress perception and signaling. This review represents the comprehensive effort to consolidate recent advances in fluorescent probe technologies, encompassing small-molecule probes, nanoprobes, and genetically encoded indicators, as revolutionary tools for deciphering stress-induced physiological dynamics. We present a comprehensive classification of fluorescent probes designed for detecting key biomolecules involved in plant stress responses, including reactive species, phytohormones, enzymes, and other signaling molecules. By critically evaluating their design principles, practical applications, and distinct advantages over conventional analytical methods, we aim to empower plant scientists in unraveling the spatiotemporal regulation of stress signaling networks. Finally, we propose strategic directions to overcome current technical bottlenecks and maximize the potential of fluorescence-based sensing in advancing sustainable agriculture.