Ozturk Rabia Yilmaz, Cakir Rabia
Department of Bioengineering, Graduate School of Science and Engineering, Yildiz Technical University, Istanbul, Türkiye.
Türkiye Biotechnology Institute, Health Institutes of Türkiye (TUSEB), Istanbul, Türkiye.
Drug Dev Ind Pharm. 2024 Sep 23:1-11. doi: 10.1080/03639045.2024.2404143.
This study assessed the anticancer activities of -loaded chitosan nanoparticles in gastric and colon cancer cells compared with fibroblast cells and examined the balance between ROS and antioxidants.
Considering this information, we synthesized -loaded chitosan nanoparticles (CO-CSNPs) the ionic gelation method. Their characterizations were carried out with ZetaSizer, UV-Vis, FTIR and SEM devices including size, morphology and surface zeta potential analysis, loading capacity, encapsulation efficiency, drug release, and chemical interactions. The anticancer activities of CO, CSNPs, and CO-CSNPs were tested against AGS, Caco-2, and normal NIH-3T3 cells using an XTT assay. The anticancer effects were evaluated using DAPI staining, scratch assay, reactive oxygen species (ROS) detection and the CUPRAC method on cellular and non-cellular processes that promote anticancer mechanisms.
Results showed that CO and CO-CNPs exhibited anticancer activity against AGS and Caco-2. Further, the formulation of CO with CSNPs enhanced the anticancer activity of CO while having no cytotoxicity on NIH-3T3. DAPI staining, scratch assay, ROS, and CUPRAC method confirmed the anticancer activity of CO and CO-CSNPs, which resulted in a reduction in the number of apoptotic cells, inhibited migration, triggered apoptotic pathway ROS, and higher antioxidant activity.
The results of the study indicate that CO-CSNPs are a promising therapeutic formulation for gastric and colon cancer treatment. We consider that this study will lead to the investigation of molecular mechanisms of CO-CSNPs in cancer treatment and their investigation in clinical studies.
本研究评估了负载药物的壳聚糖纳米颗粒在胃癌和结肠癌细胞中的抗癌活性，并与成纤维细胞进行比较，同时研究了活性氧（ROS）与抗氧化剂之间的平衡。
基于此信息，我们采用离子凝胶法合成了负载药物的壳聚糖纳米颗粒（CO-CSNPs）。使用ZetaSizer、紫外可见光谱仪、傅里叶变换红外光谱仪和扫描电子显微镜等设备对其进行表征，包括尺寸、形态和表面zeta电位分析、载药量、包封率、药物释放以及化学相互作用。使用XTT法检测CO、CSNPs和CO-CSNPs对AGS、Caco-2和正常NIH-3T3细胞的抗癌活性。通过DAPI染色、划痕试验、活性氧检测和CUPRAC法对促进抗癌机制的细胞和非细胞过程进行评估，以评价抗癌效果。
结果表明，CO和CO-CNPs对AGS和Caco-2具有抗癌活性。此外，CO与CSNPs的制剂增强了CO的抗癌活性，同时对NIH-3T3没有细胞毒性。DAPI染色、划痕试验、活性氧检测和CUPRAC法证实了CO和CO-CSNPs的抗癌活性，这导致凋亡细胞数量减少、迁移受到抑制、通过活性氧触发凋亡途径以及具有更高的抗氧化活性。
研究结果表明，CO-CSNPs是一种有前景的胃癌和结肠癌治疗制剂。我们认为，本研究将促使对CO-CSNPs在癌症治疗中的分子机制进行研究，并在临床研究中对其进行调查。
