Kleinerman E S, Ceccorulli L M, Zwelling L A, Twilley T, Herberman R B, Jacob J, Gelmann E P
J Clin Oncol. 1985 Jul;3(7):1005-12. doi: 10.1200/JCO.1985.3.7.1005.
The purpose of these studies was to determine whether peripheral blood monocytes from acquired immunodeficiency syndrome (AIDS) patients with Kaposi's sarcoma could be activated to lyse human tumor target cells in vitro. Monocytes were isolated and incubated for 24 hours in vitro with either medium (control), a crude mitogen-induced lymphokine preparation (MAF), or endotoxin before the addition of [125I]IUdR-labeled A375 melanoma target cells. Cytolysis was determined 72 hours later. Twelve (100%) of 12 patients tested had monocyte-mediated cytotoxicity values that were comparable to those of normal individuals. Recombinant human gamma interferon (IFN gamma) activated both normal and AIDS monocyte-mediated tumoricidal function only when combined with lypopolysaccharide (LPS). In addition, mononuclear cells from ten AIDS patients were also tested for their ability to secrete MAF and IFN gamma in response to a mitogenic stimulus. Lymphokines generated from all ten patients contained substantial amounts of IFN gamma (100 to 2,500 U/mL); however, three of these ten lymphokine preparations failed to activate normal monocytes to lyse tumor cells. These results suggest that monocyte-mediated tumoricidal function of AIDS patients is intact and thus suggest new approaches for the therapy of AIDS.
这些研究的目的是确定患有卡波西肉瘤的获得性免疫缺陷综合征（艾滋病）患者的外周血单核细胞在体外是否能被激活以裂解人类肿瘤靶细胞。分离单核细胞，并在添加[125I]IUdR标记的A375黑色素瘤靶细胞之前，将其在体外与培养基（对照）、粗制有丝分裂原诱导的淋巴因子制剂（巨噬细胞活化因子）或内毒素一起孵育24小时。72小时后测定细胞溶解情况。在接受检测的12名患者中，有12名（100%）的单核细胞介导的细胞毒性值与正常个体相当。仅当与脂多糖（LPS）联合使用时，重组人γ干扰素（IFNγ）才能激活正常和艾滋病患者单核细胞介导的杀肿瘤功能。此外，还检测了10名艾滋病患者的单核细胞对有丝分裂原刺激分泌巨噬细胞活化因子和IFNγ的能力。所有10名患者产生的淋巴因子都含有大量的IFNγ（100至2500 U/mL）；然而，这10种淋巴因子制剂中有3种未能激活正常单核细胞以裂解肿瘤细胞。这些结果表明，艾滋病患者单核细胞介导的杀肿瘤功能是完整的，从而为艾滋病治疗提出了新的方法。
