College of Food and Bioengineering, Henan International Joint Laboratory of Food Green Processing and Safety Control, Henan University of Science and Technology, Luoyang, 471000, PR China.
College of Food and Bioengineering, Henan International Joint Laboratory of Food Green Processing and Safety Control, Henan University of Science and Technology, Luoyang, 471000, PR China.
Talanta. 2025 Jan 1;281:126869. doi: 10.1016/j.talanta.2024.126869. Epub 2024 Sep 12.
Hydrogen sulfide (HS) is implicated in numerous physiological and pathological processes in living organisms. Abnormal levels of HS can result in various physiological disorders, highlighting the crucial need for effective identification and detection of HS at the organellar level. Although numerous HS fluorescent probes targeting organelles have been reported, a comprehensive review of these probes is required. This review focuses on the strategic selection of organelle-targeting groups and recognition sites for HS fluorescent probes. This review examines HS fluorescent probes that can specifically target lysosomes, mitochondria, endoplasmic reticulum, Golgi apparatus, and lipid droplets. These fluorescent probes have been meticulously classified and summarized based on their distinct targets, emphasizing their chemical structure, reaction mechanisms, and biological applications. We carefully designed fluorescent probes to efficiently enhance their ability to recognize target substances and exhibit significant fluorescence variations. Furthermore, we discuss the challenges inherent in the development of fluorescent probes and outline potential future directions for this exciting field.
硫化氢 (HS) 参与了生物体内许多生理和病理过程。HS 水平异常会导致各种生理紊乱，这凸显了在细胞器水平上有效识别和检测 HS 的迫切需求。尽管已经报道了许多针对细胞器的 HS 荧光探针，但需要对这些探针进行全面综述。本综述重点介绍了针对 HS 荧光探针的细胞器靶向基团和识别位点的策略选择。本综述考察了能够特异性靶向溶酶体、线粒体、内质网、高尔基体和脂滴的 HS 荧光探针。这些荧光探针根据其独特的靶标进行了精心分类和总结，强调了它们的化学结构、反应机制和生物应用。我们精心设计了荧光探针，以有效地提高它们识别目标物质的能力并表现出显著的荧光变化。此外，我们还讨论了荧光探针发展所面临的挑战，并概述了这一令人兴奋领域的潜在未来方向。
