使用合成寡核苷酸检测巢式 PCR 中由交叉污染引起的假阳性。
Use of a synthetic oligonucleotide to detect false positives caused by cross-contamination in nested PCR.
机构信息
Laboratório de Pesquisa Aplicada em Dermatologia e Infecções por Bartonela, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil; Faculty of Medicine - Endocrinology, Memorial University of Newfoundland, St. John's, Newfoundland and Labrador, Canada.
Laboratório de Pesquisa Aplicada em Dermatologia e Infecções por Bartonela, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil.
出版信息
J Microbiol Methods. 2024 Nov;226:107040. doi: 10.1016/j.mimet.2024.107040. Epub 2024 Sep 11.
Nested PCR is a useful tool for identifying low-abundance target sequences of pathogens and avoiding false negatives. However, it carries an increased risk of cross-contamination, especially with its positive control. Here, we propose using customized synthetic oligonucleotides to detect false positives due to cross-contamination.
巢式 PCR 是一种用于鉴定低丰度病原体靶序列和避免假阴性的有用工具。然而，它存在交叉污染的风险增加，尤其是阳性对照。在这里，我们建议使用定制的合成寡核苷酸来检测由于交叉污染引起的假阳性。
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