Maxamhud Sadiya, Shahiduzzaman Md, Amin A R M Beni, Hossain Md Zawad, Gentekaki Eleni, Tsaousis Anastasios D
Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, CT2 7NH Kent, UK.
Department of Parasitology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Parasitol Int. 2025 Feb;104:102967. doi: 10.1016/j.parint.2024.102967. Epub 2024 Sep 11.
The purpose of this study was to investigate the presence and transmission of Cryptosporidium spp., Blastocystis sp., Giardia intestinalis and Entamoeba histolytica between different hosts and their shared environment in a slum area of Bangladesh. A total of 102 samples were collected from Mymensingh, Bangladesh. This collection encompassed 16 human samples, 35 soil samples and 51 animal samples from various households within the region. The detection of Cryptosporidium spp., Blastocystis sp., G. intestinalis, and E. histolytica was carried out using nested PCR and/or quantitative PCR methodologies. Among the samples, 24 human, animal, and soil out of 102 were found positive for Blastocystis sp., spanning seven subtypes: ST2, ST3, ST7, ST10, ST23, ST24 and ST25. Additionally, eight samples (8/102) including human, animal and soil tested positive for Cryptosporidium spp., including C. parvum, C. baileyi, C. bovis, and C. meleagridis. Furthermore, one soil sample tested positive for G. intestinalis assemblage B, while no samples tested positive for E. histolytica. The detection of Cryptosporidium spp., Blastocystis sp., and G. intestinalis in this study has provided insights into their presence, extending beyond humans. Moreover, these findings highlight the importance of embracing a One Health perspective with an emphasis on specific parasitic microorganisms.
本研究的目的是调查孟加拉国一个贫民窟地区不同宿主及其共享环境中隐孢子虫属、芽囊原虫、肠道贾第虫和溶组织内阿米巴的存在情况及传播情况。总共从孟加拉国迈门辛采集了102份样本。这些样本包括该地区不同家庭的16份人类样本、35份土壤样本和51份动物样本。使用巢式PCR和/或定量PCR方法检测隐孢子虫属、芽囊原虫、肠道贾第虫和溶组织内阿米巴。在这些样本中，102份样本中有24份人类、动物和土壤样本被检测出芽囊原虫呈阳性，涵盖7个亚型：ST2、ST3、ST7、ST10、ST23、ST24和ST25。此外，8份样本（8/102）包括人类、动物和土壤样本检测出隐孢子虫属呈阳性，包括微小隐孢子虫、贝氏隐孢子虫、牛隐孢子虫和火鸡隐孢子虫。此外，一份土壤样本检测出肠道贾第虫B群呈阳性，而没有样本检测出溶组织内阿米巴呈阳性。本研究中隐孢子虫属、芽囊原虫和肠道贾第虫的检测结果为它们的存在情况提供了见解，其存在范围超出了人类。此外，这些发现凸显了采用“同一健康”理念并重点关注特定寄生微生物的重要性。
