Carter D P, Staehelin L A
Scan Electron Microsc. 1979(3):573-82.
Sheep red blood cell (SRBC) ghosts were incubated with preparations of anti-SRBC IgG, antigen-binding fragments (Fab') or IgG coupled to horseradish peroxidase (HRPO). Frozen samples of the labeled ghosts were deep-etched and replicated with platinum-carbon to visualize their surface features in the transmission electron microscope. An analysis of both size and density of surface "particles" observed on labeled ghosts was performed to evaluate which markers could be practically used to label these membranes. It was concluded that in this system a marker must exhibit a diameter of greater than 150 A (the apparent size of IgG-HRPO conjugates) to be consistently seen over extensive surface areas and to be distinguished from the background granularity of the SRBC ghost surface. This rules out the use of Fab' or IgG as markers in this system.
将绵羊红细胞（SRBC）空壳与抗SRBC IgG制剂、抗原结合片段（Fab'）或与辣根过氧化物酶（HRPO）偶联的IgG一起孵育。将标记后的空壳冷冻样品进行深度蚀刻，并用铂 - 碳进行复型，以便在透射电子显微镜下观察其表面特征。对标记后的空壳上观察到的表面“颗粒”的大小和密度进行分析，以评估哪些标记物可实际用于标记这些膜。得出的结论是，在该系统中，一种标记物的直径必须大于150 Å（IgG - HRPO偶联物的表观大小），才能在大面积表面上持续可见，并与SRBC空壳表面的背景颗粒度区分开来。这排除了在该系统中使用Fab'或IgG作为标记物的可能性。
