Ikeda H, Uchimura H, Greer M A
Neuroendocrinology. 1985 Jul;41(1):79-82. doi: 10.1159/000124157.
Adenohypophyseal fragments from 8 rats were perifused in small 0.2-ml chambers with medium alone or with medium containing 0.2 or 2 micrograms/dl T3 or 20 micrograms/dl T4. The TSH secretion in response to 1-min perifusion with 3 X 10(-8) M TRH was measured before and at 20- to 40-min intervals after beginning T4 or T3 perifusions. A similar temporal course of inhibition of TRH-induced TSH secretion was produced by both iodothyronines, suggesting but not proving that T4 may inhibit the TSH secretion by a direct effect not dependent on its prior intra- or extrapituitary conversion to T3.
将8只大鼠的腺垂体碎片置于0.2毫升的小室中进行灌流，灌流液为单纯培养基或含有0.2或2微克/分升T3或20微克/分升T4的培养基。在开始T4或T3灌流之前以及灌流开始后每隔20至40分钟，测量对3×10⁻⁸ M促甲状腺激素释放激素（TRH）进行1分钟灌流时促甲状腺激素（TSH）的分泌情况。两种碘甲状腺原氨酸均产生了类似的抑制TRH诱导的TSH分泌的时间进程，这表明但未证明T4可能通过不依赖其在垂体或垂体外预先转化为T3的直接作用来抑制TSH分泌。
