Gong Jishang, Li Taotao, Li Yuanfei, Xiong Xinwei, Xu Jiguo, Chai Xuewen, Ma Youji
College of Science and Technology, Gansu Agriculture University, Lanzhou 730070, China.
Institute of Biological Technology, Nanchang Normal University, Nanchang 330030, China.
Animals (Basel). 2024 Sep 5;14(17):2587. doi: 10.3390/ani14172587.
is a highly contagious Gram-positive bacterium that causes mastitis, has a high infectivity for mammary epithelial cells, and becomes challenging to treat. However, the molecular interactions between it and mammary epithelial cells remain poorly understood. This study analyzed differential gene expression in mammary epithelial cells with varying levels of infection using UID-Dual transcriptome sequencing and bioinformatics tools. This study identified 211 differentially expressed mRNAs (DEmRNAs) and 452 differentially expressed lncRNAs (DElncRNAs) in host cells, primarily enriched in anti-inflammatory responses, immune responses, and cancer-related processes. Additionally, 854 pathogen differentially expressed mRNAs (pDEmRNAs) were identified, mainly enriched in protein metabolism, gene expression, and biosynthesis processes. Mammary epithelial cells activate pathways, such as the ERK1/2 pathway, to produce reactive oxygen species (ROS) to eliminate bacteria. The bacteria disrupt the host's innate immune mechanisms by interfering with the alternative splicing processes of mammary epithelial cells. Specifically, the bacterial genes of , , and can interfere with lncRNAs targeting and in mammary epithelial cells, affecting the alternative splicing of target genes and altering normal molecular regulation.
是一种高度传染性的革兰氏阳性细菌，可引起乳腺炎，对乳腺上皮细胞具有高感染性，且治疗具有挑战性。然而，其与乳腺上皮细胞之间的分子相互作用仍知之甚少。本研究使用UID-双转录组测序和生物信息学工具分析了不同感染水平的乳腺上皮细胞中的差异基因表达。本研究在宿主细胞中鉴定出211个差异表达的mRNA（DEmRNAs）和452个差异表达的lncRNA（DElncRNAs），主要富集于抗炎反应、免疫反应和癌症相关过程。此外，还鉴定出854个病原体差异表达的mRNA（pDEmRNAs），主要富集于蛋白质代谢、基因表达和生物合成过程。乳腺上皮细胞激活诸如ERK1/2途径等通路以产生活性氧（ROS）来消除细菌。该细菌通过干扰乳腺上皮细胞的可变剪接过程来破坏宿主的固有免疫机制。具体而言，该细菌的、和基因可干扰乳腺上皮细胞中靶向和的lncRNA，影响靶基因的可变剪接并改变正常的分子调控。
