全基因组鉴定与谷子淀粉生物合成和胚乳发育相关的 microRNAs。
Genome-Wide Identification of microRNAs Associated with Starch Biosynthesis and Endosperm Development in Foxtail Millet.
机构信息
College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010000, China.
Key Laboratory of Crop Cultivation and Genetic Improvement in Inner Mongolia Autonomous Region, College of Agronomy, Inner Mongolia Agricultural University, Hohhot 010000, China.
出版信息
Int J Mol Sci. 2024 Aug 27;25(17):9282. doi: 10.3390/ijms25179282.
Foxtail millet is one of the oldest crops, and its endosperm contains up to 70% of starch. Grain filling is an important starch accumulation process associated with foxtail millet yield and quality. However, the molecular mechanisms of grain filling in foxtail millet are relatively unclear. Here, we investigate the genes and regulated miRNAs associated with starch synthesis and metabolism in foxtail millet using high-throughput small RNA, mRNA and degradome sequencing. The regulation of starch synthesis and quality is carried out mainly at the 15 DAA to 35 DAA stage during grain filling. The DEGs between waxy and non-waxy foxtail millet were significant, especially for . Additionally, ptc-miR169i_R+2_1ss21GA, fve-miR396e_L-1R+1, mtr-miR162 and PC-5p-221_23413 regulate the expression of genes associated with the starch synthesis pathway in foxtail millet. This study provides new insights into the molecular mechanisms of starch synthesis and quality formation in foxtail millet.
黍稷是最古老的作物之一，其胚乳中含有高达 70%的淀粉。籽粒灌浆是与黍稷产量和品质相关的重要淀粉积累过程。然而，黍稷灌浆的分子机制尚不清楚。在这里，我们使用高通量小 RNA、mRNA 和降解组测序研究了与黍稷淀粉合成和代谢相关的基因和调控 miRNA。淀粉合成和品质的调控主要在灌浆期的 15 DAA 到 35 DAA 阶段进行。蜡质和非蜡质黍稷之间的 DEGs 差异显著，特别是对于.此外，ptc-miR169i_R+2_1ss21GA、fve-miR396e_L-1R+1、mtr-miR162 和 PC-5p-221_23413 调节黍稷淀粉合成途径相关基因的表达。本研究为黍稷淀粉合成和品质形成的分子机制提供了新的见解。
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