Faculty of Forestry and Wood Sciences, Czech University of Life Sciences, Kamýcká 129, Suchdol, 165 21 Prague, Czech Republic.
Faculty of Science, Charles University in Prague, BIOCEV, Průmyslová 595, Vestec, 252 42 Prague, Czech Republic.
Int J Mol Sci. 2024 Sep 6;25(17):9658. doi: 10.3390/ijms25179658.
The rhizosphere is the hotspot for microbial enzyme activities and contributes to carbon cycling. Precipitation is an important component of global climate change that can profoundly alter belowground microbial communities. However, the impact of precipitation on conifer rhizospheric microbial populations has not been investigated in detail. In the present study, using high-throughput amplicon sequencing, we investigated the impact of precipitation on the rhizospheric soil microbial communities in two Norway Spruce clonal seed orchards, Lipová Lhota (L-site) and Prenet (P-site). P-site has received nearly double the precipitation than L-site for the last three decades. P-site documented higher soil water content with a significantly higher abundance of Aluminium (Al), Iron (Fe), Phosphorous (P), and Sulphur (S) than L-site. Rhizospheric soil metabolite profiling revealed an increased abundance of acids, carbohydrates, fatty acids, and alcohols in P-site. There was variance in the relative abundance of distinct microbiomes between the sites. A higher abundance of Proteobacteria, Acidobacteriota, Ascomycota, and Mortiellomycota was observed in P-site receiving high precipitation, while Bacteroidota, Actinobacteria, Chloroflexi, Firmicutes, Gemmatimonadota, and Basidiomycota were prevalent in L-site. The higher clustering coefficient of the microbial network in P-site suggested that the microbial community structure is highly interconnected and tends to cluster closely. The current study unveils the impact of precipitation variations on the spruce rhizospheric microbial association and opens new avenues for understanding the impact of global change on conifer rizospheric microbial associations.
根际是微生物酶活性的热点，对碳循环有贡献。降水是全球气候变化的重要组成部分，可以深刻改变地下微生物群落。然而，降水对针叶树根际微生物种群的影响尚未得到详细研究。在本研究中，我们使用高通量扩增子测序技术，研究了降水对两个挪威云杉克隆种子园（Lipová Lhota [L 点]和 Prenet [P 点]）根际土壤微生物群落的影响。在过去的三十年里，P 点的降水量几乎是 L 点的两倍。P 点的土壤水分含量较高，铝（Al）、铁（Fe）、磷（P）和硫（S）的丰度显著高于 L 点。根际土壤代谢物分析表明，P 点的酸、碳水化合物、脂肪酸和醇的丰度增加。两个地点之间的微生物群落相对丰度存在差异。在接收高降水的 P 点，观察到变形菌门、酸杆菌门、子囊菌门和Mortiellomycota 的丰度较高，而在接收低降水的 L 点，厚壁菌门、放线菌门、绿弯菌门、Firmicutes、Gemmatimonadota 和担子菌门的丰度较高。P 点的微生物网络的聚类系数较高，表明微生物群落结构高度互联，倾向于紧密聚类。本研究揭示了降水变化对云杉根际微生物共生体的影响，并为理解全球变化对针叶树根际微生物共生体的影响开辟了新的途径。
