College of Chinese Medicinal Materials, Jilin Agriculture University, Changchun, 130118, People's Republic of China.
National and Local Joint Engineering Research Center for Ginseng Breeding and Development, Changchun, 130118, People's Republic of China.
Sci Rep. 2022 Jul 7;12(1):11485. doi: 10.1038/s41598-022-15340-1.
Plant growth environment plays an important role in shaping soil microbial communities. To understand the response of soil rhizosphere microbial communities in Oplopanax elatus Nakai plant to a changed growth conditions from natural habitation to cultivation after transplant. Here, a comparative study of soil chemical properties and microbial community using high-throughput sequencing was conducted under cultivated conditions (CT) and natural conditions (WT), in Changbai Mountain, Northeast of China. The results showed that rhizosphere soil in CT had higher pH and lower content of soil organic matter (SOM) and available nitrogen compared to WT. These changes influenced rhizosphere soil microbial communities, resulting in higher soil bacterial and fungi richness and diversity in CT soil, and increased the relative abundance of bacterial phyla Acidobacteria, Chloroflexi, Gemmatimonadetes, Firmicutes and Patescibacteria, and the fungi phyla Mortierellomycota and Zoopagomycota, while decreased bacterial phyla Actinobacteria, WPS-2, Gemmatimonadetes, and Verrucomicrobia, and the fungi phyla Ascomycota, and Basidiomycota. Redundancy analysis analysis indicated soil pH and SOM were the primarily environmental drivers in shaping the rhizosphere soil microbial community in O. elatus under varied growth conditions. Therefore, more attention on soil nutrition management especially organic fertilizer inputs should be paid in O. elatus cultivation.
植物生长环境对土壤微生物群落的形成起着重要作用。为了了解在长白山地区，从自然栖息地到移栽后的栽培条件下，Oplopanax elatus Nakai 植物根际微生物群落对生长条件变化的响应。在这里，对栽培条件（CT）和自然条件（WT）下的土壤化学性质和微生物群落进行了高通量测序的比较研究。结果表明，与 WT 相比，CT 下的根际土壤具有更高的 pH 值和更低的土壤有机质（SOM）和有效氮含量。这些变化影响了根际土壤微生物群落，导致 CT 土壤中细菌和真菌的丰富度和多样性增加，并增加了细菌门酸杆菌门、绿弯菌门、芽单胞菌门、厚壁菌门和 Patescibacteria 的相对丰度，以及真菌门 Mortierellomycota 和 Zoopagomycota，而减少了细菌门放线菌门、WPS-2、芽单胞菌门和疣微菌门，以及真菌门子囊菌门和担子菌门。冗余分析表明，土壤 pH 值和 SOM 是影响 O. elatus 根际土壤微生物群落在不同生长条件下形成的主要环境驱动因素。因此，在 O. elatus 的栽培中，应该更加关注土壤营养管理，特别是有机肥的投入。
