Sinha Hikim A P, Chakraborty J, Jhunjhunwala J S
Urol Res. 1985;13(3):111-5. doi: 10.1007/BF00256070.
Quantitative analysis of human seminiferous epithelium was carried out using an improved method of glutaraldehyde and osmium fixation with plastic embedding. Part of each biopsy specimen was fixed in Bouin's fixative and embedded in paraffin for comparison. Epon embedded tissue had very little artifactual damage compared with paraffin embedded tissue sections. The germ cell to Sertoli cell ratios were determined by counting the various germ cells per "unit" tubular area. Data obtained by this method reflect a remarkable stability of Sertoli cell number and germ cell-Sertoli cell ratios both between biopsies from different individuals and between biopsies from right and left testes from the same individual. Agreement between the present results and those of earlier studies based on paraffin embedded testicular specimens supports the validity of this method of germ cell quantitation of human testicular biopsy samples.
采用改进的戊二醛和锇固定及塑料包埋方法对人曲细精管上皮进行定量分析。将每个活检标本的一部分固定于Bouin固定液中并石蜡包埋以作比较。与石蜡包埋组织切片相比，环氧树脂包埋的组织几乎没有人为损伤。通过计算每“单位”管状区域内的各种生殖细胞来确定生殖细胞与支持细胞的比例。通过该方法获得的数据表明，无论是在不同个体的活检之间，还是在同一个体左右睾丸活检之间，支持细胞数量以及生殖细胞与支持细胞的比例都具有显著的稳定性。本研究结果与早期基于石蜡包埋睾丸标本的研究结果一致，支持了这种对人睾丸活检样本进行生殖细胞定量分析方法的有效性。
