Kozumplík J, Sosnová J
Vet Med (Praha). 1985 Jul;30(7):385-92.
The spermiograms of 17 bulls were studied and 160 ejaculates were subjected to the thermoresistance test (38 degrees C) to evaluate sperm survival after thawing. After the first insemination of 10 682 cows, statistically significant differences were found in the fertilizing capacity of the ejaculates with various values of the thermo-resistance test. The best sperm fertilizing capacity was obtained in the ejaculates which retained progressive movement in 40% of the spermatozoa after two hours of exposure to the thermoresistance test. Out of the 1496 cows inseminated, 971 (i.e. 64.9%) got in calf, whereas after the insemination of 4216 cows with semen where only 30% of spermatozoa moved progressively at the end of the test, the number of pregnant dams was 2403, i.e. 56.98%; this difference is statistically significant (p0.05). At a lower sperm activity in the test the fertility after the first insemination was even lower. Although there was some difference in the individual fertility of bulls (54 to 67%), a positive relationship between the results of the thermoresistance test and fertility was recorded in all bulls.
对17头公牛的精液样本进行了研究，并对160份射精样本进行了耐热性测试（38摄氏度），以评估解冻后的精子存活率。在对10682头母牛进行首次人工授精后，发现耐热性测试值不同的射精样本在受精能力上存在统计学显著差异。在经过两小时耐热性测试后仍有40%的精子保持前进运动的射精样本中，精子的受精能力最佳。在接受人工授精的1496头母牛中，有971头（即64.9%）受孕，而在对4216头母牛进行人工授精时，使用的精液在测试结束时只有30%的精子能够前进运动，受孕母牛数量为2403头，即56.98%；这一差异具有统计学显著性（p<0.05）。在测试中精子活力较低时，首次人工授精后的受孕率更低。尽管公牛个体的受孕率存在一定差异（54%至67%），但在所有公牛中均记录到耐热性测试结果与受孕率之间存在正相关关系。
