基于智能手机和水凝胶的大肠杆菌 O157:H7 和金黄色葡萄球菌的便携式实时检测
Portable real-time determination of Escherichia coli O157:H7 and Staphylococcus aureus based on smartphones and hydrogels.
机构信息
College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Jinzhou, Liaoning 121013, China.
College of Food Science and Technology, Bohai University, National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products, Food Safety Key Lab of Liaoning Province, Institute of Ocean Research, The Fresh Food Storage and Processing Technology Research Institute of Liaoning Provincial Universities, Jinzhou, Liaoning 121013, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Jan 15;325:125119. doi: 10.1016/j.saa.2024.125119. Epub 2024 Sep 13.
The aptamers functionalized orange-emission carbon dots (OCDs) and green-emission carbon dots (GCDs) had dual-emission peaks with single excitation. Tungsten disulfide nanosheets (WS NSs)-triggered fluorescence quenching achieved the ratiometric fluorescence determination of Escherichia coli O157:H7 (E. coli O157:H7) and Staphylococcus aureus (S. aureus) with wide ranges of 18-1.8 × 10 and 37-3.7 × 10 CFU/mL and low detection limits of 8 and 20 CFU/mL, respectively. The results in real sample with recoveries of 90-101 % and RSD < 4.12 % were no significant difference from standard plate counting method. Meanwhile, the dual-color CDs were further adopted in the smartphone-assisted hydrogel platform and achieved speedy, sensitive, portable and real-time determination of E. coli O157:H7 and S. aureus in real samples. This work has not only developed ratiometric fluorescence detection and constructed a portable hydrogel platform, but also provided a unique strategy in developing a time-efficient and easy-to-use portable device in food safety monitoring.
适配体功能化的橙红色发射碳点（OCDs）和绿色发射碳点（GCDs）具有单激发双发射峰。二硫化钨纳米片（WS NSs）触发的荧光猝灭实现了大肠杆菌 O157:H7（E. coli O157:H7）和金黄色葡萄球菌（S. aureus）的比率荧光测定，其范围分别为 18-1.8×10 和 37-3.7×10 CFU/mL，检测限分别为 8 和 20 CFU/mL。在实际样品中的结果与标准平板计数法相比没有显著差异，回收率为 90-101%，RSD<4.12%。同时，双色 CDs 进一步被用于智能手机辅助水凝胶平台，实现了对实际样品中大肠杆菌 O157:H7 和金黄色葡萄球菌的快速、灵敏、便携和实时测定。这项工作不仅开发了比率荧光检测并构建了便携式水凝胶平台，而且为开发用于食品安全监测的高效、易用的便携式设备提供了独特的策略。
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