Dong Ruihong, Leng Tuo, Wang Yuting, Gan Bei, Yu Qiang, Xie Jianhua, Du Qianwen, Zhu Mengting, Chen Yi
State Key Laboratory of Food Science and Resources, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
Jiangxi Provincial Product Quality Supervision Testing College, Nanchang 330029, People's Republic of China.
Food Chem. 2025 Jan 15;463(Pt 2):141217. doi: 10.1016/j.foodchem.2024.141217. Epub 2024 Sep 12.
To identify chemical-markers from hot-pressed, cold-pressed, organic-solvent, aqueous-enzymatic and water extracted camellia oils (HPO, CPO, OSO, AEO, WEO). We report a full composition-wide association study based on GC-MS, LC-MS and HNMR. Squalene, β-amyrin and lupeol were potential-markers in distinguishing different oils through GC-MS. Naringenin, FA 18:1 + 10, undecanedioic acid and tridecanedioic acid exhibited were up-regulated in HPO. 16-Hydroxyhexadecanoic acid, octadecanoic acid and 9-hydroxyoctadecadienoic acid were potential-metabolites in CPO. Characteristic-markers in WEO were hydroquinidine and undecanedioic acid. Gallic acid, hydroquinidine, lichesterylic acid and 7,4'-dihydroxyflavone were biomarkers in AEO. Oleic acid, linoleic acid and triacylglycerols may be potential key markers to distinguish AEO from others via HNMR. Finally, Naringenin, gallic acid, kaempferol, 7,4'-dihydroxyflavone, (Z)-5,8,11-trihydroxyoctadec-9-enoic acid and β-amyrin were screened and validate through integration of nonglyceride minor components and trace metabolites. Results provided understanding of chemical diversity for different processed-camellia oils, and proposed a complementary strategy to distinguish different camellia oils for multidimensional perspective.
为了从热榨、冷榨、有机溶剂萃取、水酶法萃取和水萃取的山茶油（HPO、CPO、OSO、AEO、WEO）中鉴定化学标志物。我们报告了一项基于气相色谱-质谱联用（GC-MS）、液相色谱-质谱联用（LC-MS）和核磁共振氢谱（HNMR）的全成分关联研究。角鲨烯、β-香树脂醇和羽扇豆醇是通过GC-MS区分不同油类的潜在标志物。柚皮素、FA 18:1 + 10、十一烷二酸和十三烷二酸在HPO中上调。16-羟基十六烷酸、十八烷酸和9-羟基十八碳二烯酸是CPO中的潜在代谢物。WEO中的特征性标志物是氢化奎尼丁和十一烷二酸。没食子酸、氢化奎尼丁、地衣硬脂酸和7,4'-二羟基黄酮是AEO中的生物标志物。油酸、亚油酸和三酰甘油可能是通过HNMR将AEO与其他油类区分开来的潜在关键标志物。最后，通过整合非甘油酯微量成分和痕量代谢物，筛选并验证了柚皮素、没食子酸、山奈酚、7,4'-二羟基黄酮、(Z)-5,8,11-三羟基十八碳-9-烯酸和β-香树脂醇。研究结果有助于理解不同加工山茶油的化学多样性，并从多维角度提出了一种区分不同山茶油的补充策略。
