制备抗山奈酚的单克隆抗体及其在山奈酚和橙皮苷酶联免疫吸附测定中的应用。
Preparation of monoclonal antibody against rhoifolin and its application in enzyme-linked immunosorbent assay of rhoifolin and diosmin.
机构信息
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, China; National Citrus Engineering Research Center, Chongqing, 400712, China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, China; National Citrus Engineering Research Center, Chongqing, 400712, China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
出版信息
Talanta. 2025 Jan 1;281:126871. doi: 10.1016/j.talanta.2024.126871. Epub 2024 Sep 11.
Both rhoifolin and diosmin belong to flavonoids, which are widely present in citrus. Diosmin is not only used in the medical field in the world, but also used as a dietary supplement in the United States. Rhoifolin has a similar structure to diosmin and also exhibits antioxidant and anti-inflammatory properties. In this study, an anti-rhoifolin monoclonal antibody was prepared and an indirect competitive enzyme-linked immunosorbent assay (icELISA) method was established. The half-maximal inhibitory concentration (IC) of icELISA was determined to be 4.83 ng/mL, and the detection range was 0.97-33.87 ng/mL. The results of UPLC-MS/MS and icELISA generally demonstrate consistency. Moreover, by exploiting the cross-reactivity of the antibody, diosmin in tablets can be detected by icELISA. The results demonstrate that the developed method has good accuracy, reproducibility, and broad application prospects.
二氢槲皮素和地奥司明都属于黄酮类化合物，广泛存在于柑橘类水果中。地奥司明不仅在世界医学领域得到应用，而且还被用作美国的膳食补充剂。二氢槲皮素的结构与地奥司明相似，也具有抗氧化和抗炎特性。本研究制备了抗二氢槲皮素单克隆抗体，并建立了间接竞争酶联免疫吸附测定（icELISA）方法。icELISA 的半抑制浓度（IC）为 4.83ng/mL，检测范围为 0.97-33.87ng/mL。UPLC-MS/MS 和 icELISA 的结果总体上具有一致性。此外，利用抗体的交叉反应性，icELISA 可检测片剂中的地奥司明。结果表明，所开发的方法具有良好的准确性、重现性和广阔的应用前景。
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