Balaban Emre, Demir Emine, Çelebi Erdivanlı Özlem, Mercantepe Tolga, Gökçe Fatih Mehmet, Tümkaya Levent, Dursun Engin
Recep Tayyip Erdogan University Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Rize, Turkey.
Private Clinic, İstanbul, Turkey.
J Stomatol Oral Maxillofac Surg. 2025 Mar;126(2):102071. doi: 10.1016/j.jormas.2024.102071. Epub 2024 Sep 12.
To evaluate the effect of concentrated growth factor (CGF) on regeneration of facial nerve after crush injury.
Fourteen rats were randomized into two groups. The control group (CG) (n = 7) received a crush injury to the right facial nerve. The CGF group (CGFG) (n = 7) received a crush injury to the right facial nerve and concentrated growth factor prepared from their own blood thereafter. Left facial nerves were used for functional comparison. Nerve function was evaluated using whisker movements and electromyography. Histologic properties were evaluated using hematoxylin and eosin and Masson-trichrome staining, and immunohistochemical properties were evaluated using Neurofilament-H and Anti-Tau degeneration markers.
In the CGFG, whisker functions began to recover earlier and recovered more quickly compared with the CG. The CG showed significantly prolonged latency and reduced amplitudes in the first week compared with the CGFG (p < 0.05). Recordings of 4th-week latency and amplitudes were similar to the preoperative period in the CGFG (p > 0.05), whereas recordings of the same week were significantly worse in the CG (p < 0.05). Edema and fibrosis were also more pronounced in the CG compared with the CGFG. Neurofilament-H and Anti-Tau were at significantly high levels in the CG (p < 0.05).
Concentrated growth factor promotes recovery in facial crush injury and may prove a cost-effective, practical, and effective treatment choice in peripheral nerve injury.
评估浓缩生长因子（CGF）对挤压伤后面神经再生的影响。
将14只大鼠随机分为两组。对照组（CG）（n = 7）右侧面神经接受挤压伤。CGF组（CGFG）（n = 7）右侧面神经接受挤压伤，之后注射由其自身血液制备的浓缩生长因子。左侧面神经用于功能对照。通过触须运动和肌电图评估神经功能。使用苏木精-伊红染色和Masson三色染色评估组织学特性，使用神经丝-H和抗tau蛋白变性标记物评估免疫组化特性。
与CG组相比，CGFG组触须功能恢复更早且更快。与CGFG组相比，CG组在第一周潜伏期显著延长，波幅降低（p < 0.05）。CGFG组第4周潜伏期和波幅记录与术前相似（p > 0.05），而CG组同一周记录明显更差（p < 0.05）。与CGFG组相比，CG组水肿和纤维化也更明显。CG组神经丝-H和抗tau蛋白水平显著升高（p < 0.05）。
浓缩生长因子可促进面部挤压伤恢复，可能是一种治疗周围神经损伤经济有效、切实可行的治疗选择。
