College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei 071000, China.
College of Food Science and Technology, Hebei Agricultural University, Baoding, Hebei 071000, China; College of Biochemistry and Environmental Engineering, Baoding University, Baoding, Hebei 071000, China.
Food Res Int. 2024 Nov;195:114976. doi: 10.1016/j.foodres.2024.114976. Epub 2024 Aug 23.
Changes in dietary patterns and living habits have led to an increasing number of individuals with elevated cholesterol levels. Excessive consumption of high-cholesterol foods can disrupt the body's lipid metabolism. Numerous studies have firmly established the cholesterol-lowering effects of probiotics and prebiotics, with evidence showing that the synergistic use of synbiotics is functionally more potent than using probiotics or prebiotics alone. Currently, the screening strategy involves screening prebiotics for synbiotic development with probiotics as the core. However, in comparison to probiotics, there are fewer types of prebiotics available, leading to limited resources. Consequently, the combinations of synbiotics obtained are restricted, and probiotics and prebiotics are only relatively suitable. Therefore, in this study, a novel synbiotic screening strategy with prebiotics as the core was developed. The synbiotic combination of Lactobacillus rhamnosus S_82 and xylo-oligosaccharides was screened from the intestinal tract of young people through five generations of xylo-oligosaccharides. Subsequently, the cholesterol-lowering ability of the medium was simulated, and the two carbon sources of glucose and xylo-oligosaccharides were screened out. The results showed that synbiotics may participate in cholesterol-lowering regulation by down-regulating the expression of NPC1L1 gene, down-regulating ACAT2 and increasing the expression of ABCG8 gene in vitro through cell adsorption and cell absorption in vitro, and regulating the intestinal microbiota. Synbiotics hold promise as potential candidates for the prevention of hypercholesterolemia in humans and animals, and this study providing a theoretical foundation for the development of new synbiotic products.
饮食习惯和生活习惯的改变导致越来越多的人胆固醇水平升高。过量摄入高胆固醇食物会破坏身体的脂质代谢。大量研究已经证实了益生菌和益生元的降胆固醇作用，有证据表明，合生元的协同使用在功能上比单独使用益生菌或益生元更有效。目前，筛选策略涉及以益生菌为核心筛选用于合生元开发的益生元。然而，与益生菌相比，可用的益生元种类较少，导致资源有限。因此，获得的合生元组合受到限制，益生菌和益生元只是相对适用。因此，在本研究中，开发了一种以益生元为核心的新型合生元筛选策略。通过五轮木低聚糖筛选出来源于年轻人肠道的鼠李糖乳杆菌 S_82 和木低聚糖的合生元组合。随后，模拟了该合生元组合的降胆固醇能力，并筛选出葡萄糖和木低聚糖这两种碳源。结果表明，合生元可能通过下调 NPC1L1 基因的表达、下调 ACAT2 和增加 ABCG8 基因的表达以及调节肠道微生物群，参与胆固醇的调节。合生元有望成为预防人类和动物高胆固醇血症的潜在候选物，本研究为新型合生元产品的开发提供了理论基础。
