Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, School of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, China; Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, School of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, China.
Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, School of Life Sciences, Huzhou University, Huzhou, Zhejiang 313000, China; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China.
Pestic Biochem Physiol. 2024 Sep;204:106105. doi: 10.1016/j.pestbp.2024.106105. Epub 2024 Aug 28.
The invasive golden apple snail Pomacea canaliculata is one of the devastating threats to aquatic ecosystems and wetland agriculture worldwide. Macrolides from microbes display various advantages over other compounds in controlling snails. However, emergence of antibiotic-resistant phenotypes against certain macrolides in the field appeals for exploring more effectively molluscicidal macrolides. Here, two borrelidins, borrelidin BN1 and BN2, from the extract of a Streptomyces strain fermentation were evaluated for molluscicidal potential against P. canaliculata using both immersion and contact bioassay methods. Borrelidin BN1 (borrelidin A) presented a significant molluscicidal activity comparable to the chemical pesticide metaldehyde, and had a much lower median lethal concentration value (LC, 522.984 μg·ml) than avermectin B1 at 72 h of contact-killing treatment. Snail growth was inhibited by borrelidin BN1 more than by metaldehyde at sublethal concentrations, consistent with responses of key biochemical parameters. Exposure to borrelidin BN1 decreased the activity of acetylcholinesterase (AChE), glutathione S-transferase (GST), aspartate aminotransferase (AST), alanine aminotransferase (ALT) as well as the levels of energy reserves and sex steroids in snail tissues, while increased the activity of superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH) and the level of lipid peroxidation (LPO). Further application assay confirmed that borrelidin BN1 protected crop plant Zizania latifolia from P. canaliculata damage via suppressing snail population density. These findings suggest great potential of borrelidin BN1 as a molluscicide. Additionally, its higher activity than the stereoisomeric borrelidin BN2 (borrelidin F) implied better molluscicidal borrelidins could be acquired through structural optimization.
入侵性的金黄苹果螺 Pomacea canaliculata 是对全球水生生态系统和湿地农业具有破坏性的威胁之一。微生物来源的大环内酯类化合物在控制蜗牛方面显示出比其他化合物更多的优势。然而，田间对抗生素产生抗性的表型对某些大环内酯类化合物的出现呼吁探索更有效的杀螺大环内酯类化合物。在这里，从一株链霉菌发酵提取物中评估了两种布雷利丁（borrelidin BN1 和 BN2）对金黄苹果螺的杀螺潜力，采用浸泡和接触生物测定方法。布雷利丁 BN1（布雷利丁 A）表现出与化学农药灭多威相当的显著杀螺活性，其半致死浓度值（LC）比阿维菌素 B1 在 72 小时接触致死处理时低得多（LC522.984μg·ml）。在亚致死浓度下，布雷利丁 BN1 对蜗牛生长的抑制作用大于灭多威，这与关键生化参数的反应一致。暴露于布雷利丁 BN1 会降低蜗牛组织中乙酰胆碱酯酶（AChE）、谷胱甘肽 S-转移酶（GST）、天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）的活性以及能量储备和性类固醇的水平，同时会增加超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、乳酸脱氢酶（LDH）的活性和脂质过氧化（LPO）的水平。进一步的应用试验证实，布雷利丁 BN1 通过抑制蜗牛种群密度来保护作物植物菰免受金黄苹果螺的损害。这些发现表明布雷利丁 BN1 作为一种杀螺剂具有巨大的潜力。此外，其活性高于立体异构体布雷利丁 BN2（布雷利丁 F）表明通过结构优化可以获得更高活性的杀螺布雷利丁。
