Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China.
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Sci Data. 2024 Sep 14;11(1):1002. doi: 10.1038/s41597-024-03846-4.
Brontispa longissima is a highly destructive pest that affects coconut and ornamental palm plants. It is widely distributed across Southeast and East Asia and the Pacific region, causing production losses of up to 50-70%. While control methods and ecological phenomena have been the primary focus of research, there is a significant lack of studies on the molecular mechanisms underlying these ecological phenomena. The absence of a reference genome has also hindered the development of new molecular-targeted control technologies. In this study, we conducted a karyotype analysis of B. longissima and assembled the first high-quality chromosome-level genome. The assembled genome is 582.24 Mb in size, with a scaffold N50 size of 63.81 Mb, consisting of 10 chromosomes and a GC content of 33.71%. The BUSCO assessment indicated a completeness estimate of 98.1%. A total of 23,051 protein-coding genes were predicted. Our study provides a valuable genomic resource for understanding the mechanisms of adaptive evolution and facilitates the development of new molecular-targeted control methods for B. longissima.
椰心叶甲是一种极具破坏性的害虫，会危害椰子和观赏棕榈植物。它广泛分布于东南亚和东亚以及太平洋地区，会导致高达 50-70%的产量损失。虽然控制方法和生态现象一直是研究的重点，但对这些生态现象背后的分子机制的研究却非常少。缺乏参考基因组也阻碍了新的分子靶向控制技术的发展。在本研究中，我们对椰心叶甲进行了染色体核型分析，并组装了第一个高质量的染色体水平基因组。组装的基因组大小为 582.24 Mb，支架 N50 大小为 63.81 Mb，由 10 条染色体组成，GC 含量为 33.71%。BUSCO 评估表明完整性估计值为 98.1%。共预测到 23051 个蛋白质编码基因。我们的研究为了解适应性进化的机制提供了有价值的基因组资源，并有助于开发针对椰心叶甲的新的分子靶向控制方法。
