Department of Critical Care Medicine, Maoming People's Hospital, Maoming, 525000, Guangdong, China.
Department of Food Science and Engineering, Jinan University, Guangzhou, 510632, Guangdong, China.
Sci Rep. 2024 Sep 14;14(1):21504. doi: 10.1038/s41598-024-72797-y.
WSGP has demonstrated significant potential for various bioactive effects. However, limited research has explored their anti-ulcerative colitis (UC) effects and mechanism on the colonic system and gut microbial metabolites. We evaluated the ameliorative effects of WSGP on the UC mice model. Using H&E to assess histological injury of colon morphology, AB-PAS staining to detect mucin secretion from goblet cells and the mucous layer, IF to evaluate the expression of intercellular tight junction proteins, ELISA to measure inflammatory factors, WB analysis to measure protein expression of inflammatory signaling pathways, RT-qPCR to quantify gene transcription of inflammatory factors, and LC-MS to analyze metabolites in mouse cecum contents. WSGP supplementation increased food intake, body weight, and colon length while reducing disease activity and histological scores in colitis-afflicted mice. WSGP mitigated colonic tissue damage and restored intestinal barrier integrity by suppressing NF-κB/STAT3 signaling, thereby decreasing gene transcription, protein expression of proinflammatory factors, and nitric oxide production. Additionally, WSGP improved UC by altering the variety of intestinal microbial metabolites. This study demonstrates that WSGP supplementation attenuates UC mice by suppressing the NF-κB/STAT3 signaling pathway, enhancing mucosal barrier function, reducing pro-inflammatory cytokines, and modulating gut microbial metabolites.
WSGP 表现出对各种生物活性作用的巨大潜力。然而，有限的研究探索了其对溃疡性结肠炎（UC）的作用机制和对结肠系统及肠道微生物代谢物的影响。我们评估了 WSGP 对 UC 小鼠模型的改善作用。使用 H&E 评估结肠形态的组织学损伤，AB-PAS 染色检测杯状细胞的粘蛋白分泌和粘液层，IF 评估细胞间紧密连接蛋白的表达，ELISA 测量炎症因子，WB 分析测量炎症信号通路的蛋白表达，RT-qPCR 定量炎症因子的基因转录，LC-MS 分析小鼠盲肠内容物中的代谢物。WSGP 补充剂增加了食物摄入、体重和结肠长度，同时降低了结肠炎小鼠的疾病活动度和组织学评分。WSGP 通过抑制 NF-κB/STAT3 信号通路减轻结肠组织损伤并恢复肠道屏障完整性，从而降低基因转录、促炎因子的蛋白表达和一氧化氮的产生。此外，WSGP 通过改变肠道微生物代谢物的种类来改善 UC。本研究表明，WSGP 补充剂通过抑制 NF-κB/STAT3 信号通路、增强粘膜屏障功能、减少促炎细胞因子和调节肠道微生物代谢物来减轻 UC 小鼠的病情。
