Removal of aflatoxin M in milk using magnetic laccase/MoS/chitosan nanocomposite as an efficient sorbent.

The current study tries to find the impact of the integration of laccase enzyme (Lac) onto magnetized chitosan (Cs) nanoparticles composed of molybdenum disulfide (MoS NPs) (FeO/Cs/MoS/Lac NPs) on the removal of AFM in milk samples. The FeO/Cs/MoS/Lac NPs were characterized by FT-IR, XRD, BET, TEM, FESEM, EDS, PSA, and VSM analysis. The cytotoxic activity of the synthesized nanoparticles in different concentrations was evaluated using the MTT method. The results show that the synthesized nanoparticles don't have cytotoxic activity at concentrations less than 20 mg/l. The ability of the prepared nanoparticles to remove AFM was compared by bare laccase enzyme, MoS, and FeO/Cs/MoS composite, indicating that the FeO/Cs/MoS/Lac NPs the highest adsorption efficiency toward AFM. Besides, the immobilization efficiency of laccase with a concentration range of 0.5-2.0 was investigated, indicating that the highest activity recovery of 96.8% was obtained using 2 mg/ml laccase loading capacity. The highest removal percentage of AFM (68.5%) in the milk samples was obtained by the FeO/Cs/MoS/Lac NPs at a contact time of 1 h. As a result, FeO/MoS/Cs/Lac NPs can potentially be utilized as an effective sorbent with high capacity and selectivity to remove AFM from milk samples.