Balani S K, Yeh H J, Ryan D E, Thomas P E, Levin W, Jerina D M
Biochem Biophys Res Commun. 1985 Jul 31;130(2):610-6. doi: 10.1016/0006-291x(85)90460-7.
The absolute configurations of the enantiomeric 5,6-arene oxides of 7,12-dimethylbenz[a]anthracene (DMBA) were recently assigned such that the late eluting enantiomer from a chiral HPLC column has 5R,6S absolute configuration. [Mushtaq et al. (1984) BBRC 125, 539]. The authors further concluded that the 5R,6S-enantiomer predominates on metabolism of DMBA by cytochrome P450c in liver microsomes from 3-methylcholanthrene-treated rats. Their chemical assignment of absolute configuration is incorrect. Thus, metabolism of DMBA by these microsomes as well as by homogeneous cytochrome P450c produces 5,6-oxide highly enriched (95%) in the 5S,6R-enantiomer in accord with theoretical predictions.
最近确定了7,12-二甲基苯并[a]蒽（DMBA）对映体5,6-芳烃氧化物的绝对构型，使得手性高效液相色谱柱上洗脱较晚的对映体具有5R,6S绝对构型。[Mushtaq等人（1984年）《生物化学与生物物理研究通讯》125, 539]。作者进一步得出结论，在经3-甲基胆蒽处理的大鼠肝微粒体中，细胞色素P450c对DMBA进行代谢时，5R,6S-对映体占主导。他们对绝对构型的化学指定是错误的。因此，这些微粒体以及均一的细胞色素P450c对DMBA的代谢产生的5,6-氧化物中，5S,6R-对映体高度富集（95%），这与理论预测一致。
