Kulmacz R J, Miller J F, Lands W E
Biochem Biophys Res Commun. 1985 Jul 31;130(2):918-23. doi: 10.1016/0006-291x(85)90504-2.
Reaction conditions which promote the heme-dependent peroxidase activity of prostaglandin H synthase appear to stimulate the heme-dependent cyclooxygenase activity also present in the synthase, even though the cyclooxygenase requires hydroperoxide for activity. However, aspirin-treated synthase, which retains only peroxidase activity, inhibited the cyclooxygenase activity of untreated synthase in the manner observed with similar levels of glutathione peroxidase. Any stimulatory effect of the synthase peroxidase on the synthase cyclooxygenase is thus likely to involve an intramolecular mechanism. Participation of peroxidase intermediates (Compounds I and II) in the initiation of a cyclooxygenase free radical chain reaction may provide an intramolecular mechanism for stimulation of the synthase cyclooxygenase by the synthase peroxidase.
促进前列腺素H合酶血红素依赖性过氧化物酶活性的反应条件似乎也会刺激合酶中同样存在的血红素依赖性环氧化酶活性，尽管环氧化酶的活性需要氢过氧化物。然而，仅保留过氧化物酶活性的阿司匹林处理的合酶，以与类似水平的谷胱甘肽过氧化物酶观察到的方式抑制未处理合酶的环氧化酶活性。因此，合酶过氧化物酶对合酶环氧化酶的任何刺激作用可能涉及分子内机制。过氧化物酶中间体（化合物I和II）参与环氧化酶自由基链反应的引发，可能为合酶过氧化物酶刺激合酶环氧化酶提供分子内机制。
