Carsten M E, Miller J D
Biochem Biophys Res Commun. 1985 Aug 15;130(3):1027-31. doi: 10.1016/0006-291x(85)91718-8.
Microsomes derived from pregnant uterine sarcoplasmic reticulum, isolated by differential and sucrose density gradient centrifugation, accumulates Ca2+ in the presence of ATP. Inositol trisphosphate caused release of this Ca2+, in a dose dependent manner. 40% of the Ca2+ that can be released by the ionophore A23187 was released by 5 microM inositol trisphosphate. Removal of Mg by EDTA prior to addition of inositol trisphosphate did not change the course of Ca2+ release. These results indicate that by mobilizing intracellular Ca2+, inositol trisphosphate may be the link between hormonal stimuli and smooth muscle contraction.
通过差速离心和蔗糖密度梯度离心分离得到的来自妊娠子宫肌浆网的微粒体，在ATP存在的情况下积累Ca2+。肌醇三磷酸以剂量依赖的方式引起这种Ca2+的释放。5 microM肌醇三磷酸释放出了离子载体A23187所能释放的40%的Ca2+。在添加肌醇三磷酸之前用EDTA去除Mg并没有改变Ca2+释放的过程。这些结果表明，通过动员细胞内Ca2+，肌醇三磷酸可能是激素刺激与平滑肌收缩之间的联系。
