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Investigation of rhodopsin catalyzed G-protein GTP-binding using [35S] GTP gamma S--effects of regeneration and hydroxylamine.

作者信息

Cook N J, Pellicone C, Virmaux N

出版信息

Biochem Int. 1985 Apr;10(4):647-53.

PMID:3927920
Abstract

A simple reconstitution system for studying rhodopsin-catalyzed G-protein GTP-binding is described. Purified rhodopsin is recombined with a G-protein containing extract in the presence of [35S]GTP gamma S and after incubation the reaction is stopped by rapid cooling and filtration. The system has been used to study the effects of 11-cis-retinal and hydroxylamine on R*-catalyzed G-protein GTP-binding. Since both these compounds greatly reduced binding, our results provide further evidence that it is the rhodopsin intermediate metarhodopsin II which interacts with G-protein.

摘要

相似文献

1
Investigation of rhodopsin catalyzed G-protein GTP-binding using [35S] GTP gamma S--effects of regeneration and hydroxylamine.
Biochem Int. 1985 Apr;10(4):647-53.
2
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[Molecular mechanisms of photoreception. IV. Photoregeneration of rhodopsin from metarhodopsin II using the artificial lipid membrane method for detection of intermediate steps of this reaction].[光感受器的分子机制。IV. 使用人工脂质膜方法检测视紫红质从变视紫红质II再生的中间步骤，以实现视紫红质的光再生]
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Eur J Biochem. 1983 Nov 15;136(3):489-99. doi: 10.1111/j.1432-1033.1983.tb07767.x.

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