Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
School of Dentistry, Institute of Clinical Sciences, College of Medical and Dental Sciences, University of Birmingham, 5, Mill Pool Way, Edgbaston, Birmingham, B5 7EG, United King-dom .
Swiss Dent J. 2024 Sep 9;134(4):1-13. doi: 10.61872/sdj-2024-04-01.
Endodontic irrigation protocols are not only used to clean and disinfect the root canal system, but also to condition the canal wall dentine for subsequent root filling. In this study we tested whether a final irrigation step with saline solution or 80% ethanol improved root canal sealabilty by two popular sealers, an epoxy resin (AH Plus) and a hydraulic calcium silicate cement-based product (BioRoot RCS). Root canals in extracted single-rooted human teeth were instrumented and filled with a matched gutta-percha cone and sealer. During instrumentation and prior to root filling, sealer-specific irrigation protocols were applied. These involved a combined sodium hypochlorite/1-hydroxyethylidene-1,1-diphosphonic acid application, which was followed by irrigation with ethylenediaminetetraacetic acid (EDTA) for AH Plus. Protocols were followed by a 5-ml ultimate rinse with saline solution or 80% ethanol. No such final rinse was the control (N = 9). Canals were then dried with matched paper points. One week after root filling and storage of the teeth at 37°C in a humid environment, Rhodamine B was used to trace leakage. Two-way ANOVA revealed that the type of sealer had a significant (P < 0.05) impact on apical dye penetration while the final rinse did not (P > 0.05). AH Plus provided the slightly better seal (P < 0.05). Leakage occurred between the sealer and the dentin with AH Plus, and between the sealer-to-dentin as well as the sealer-to-gutta-percha interface with BioRoot RCS. In summary and under current conditions, there was no benefit from applying saline or ethanol as an ultimate rinsing solution prior to drying the canal with matched paper points.
牙髓冲洗方案不仅用于清洁和消毒根管系统，还用于调理根管壁牙本质，为后续的根管充填做准备。在这项研究中，我们测试了用生理盐水或 80%乙醇进行最终冲洗步骤是否可以提高两种流行的密封剂（环氧树脂 AH Plus 和水硬性硅酸钙基产品 BioRoot RCS）的根管密封性。我们对离体单根人牙的根管进行了器械处理并填充了匹配的牙胶尖和密封剂。在器械处理和根管充填之前，应用了特定于密封剂的冲洗方案。这些方案包括次氯酸钠/1-羟乙基二膦酸的联合应用，随后用乙二胺四乙酸（EDTA）冲洗 AH Plus。方案之后用 5 毫升生理盐水或 80%乙醇进行最终冲洗。不进行最终冲洗作为对照（N = 9）。然后用匹配的纸尖吸干根管。在 37°C 的潮湿环境中储存牙齿一周后，使用罗丹明 B 追踪渗漏。双向方差分析显示，密封剂的类型对根尖染料渗透有显著影响（P < 0.05），而最终冲洗没有影响（P > 0.05）。AH Plus 提供了稍微更好的密封（P < 0.05）。AH Plus 与牙本质之间以及 BioRoot RCS 与牙本质之间以及密封剂与牙胶尖之间均发生渗漏。总之，在当前条件下，在用匹配的纸尖吸干根管之前，用生理盐水或乙醇作为最终冲洗溶液并没有益处。
