在2型糖尿病中，cNPAS2通过调节KANK1表达诱导β细胞功能障碍。
cNPAS2 induced β cell dysfunction by regulating KANK1 expression in type 2 diabetes.
作者信息
Yin Yan-Bin, Ji Wei, Liu Ying-Lan, Gao Qian-Hao, He Dong-Dong, Xu Shi-Lin, Fan Jing-Xin, Zhang Li-Hai
机构信息
Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China.
Department of Anesthesiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264199, Shandong Province, China.
出版信息
World J Diabetes. 2024 Sep 15;15(9):1932-1941. doi: 10.4239/wjd.v15.i9.1932.
BACKGROUND
Diabetes mellitus type 2 (T2DM) is formed by defective insulin secretion with the addition of peripheral tissue resistance of insulin action. It has been affecting over 400 million people all over the world.
AIM
To explore the pathogenesis of T2DM and to develop and implement new prevention and treatment strategies for T2DM.
METHODS
Receiver operating characteristic (ROC) curve analysis was used to conduct diagnostic markers. The expression level of genes was determined by reverse transcription-PCR as well as Western blot. Cell proliferation assays were performed by cell counting kit-8 (CCK-8) tests. At last, T2DM mice underwent Roux-en-Y gastric bypass surgery.
RESULTS
We found that was significantly up-regulated in islet β cell apoptosis of T2DM. The ROC curve revealed that was capable of accurately diagnosing T2DM. overexpression did increase the level of . In addition, the CCK-8 test revealed knocking down NPAS2 and KANK1 increased the proliferation of MIN6 cells. At last, we found that gastric bypass may treat type 2 diabetes by down-regulating and .
CONCLUSION
This study demonstrated that induced β cell dysfunction by regulating expression in type 2 diabetes, and it may be an underlying therapy target of T2DM.
背景
2型糖尿病（T2DM）是由胰岛素分泌缺陷以及胰岛素作用的外周组织抵抗共同形成的。它已经影响了全球超过4亿人。
目的
探讨T2DM的发病机制，并开发和实施T2DM的新预防和治疗策略。
方法
采用受试者工作特征（ROC）曲线分析来进行诊断标志物研究。通过逆转录聚合酶链反应（RT-PCR）以及蛋白质免疫印迹法（Western blot）测定基因的表达水平。采用细胞计数试剂盒-8（CCK-8）试验进行细胞增殖测定。最后，对T2DM小鼠进行Roux-en-Y胃旁路手术。
结果
我们发现，在T2DM的胰岛β细胞凋亡中显著上调。ROC曲线显示，能够准确诊断T2DM。的过表达确实增加了的水平。此外，CCK-8试验显示，敲低NPAS2和KANK1可增加MIN6细胞的增殖。最后，我们发现胃旁路手术可能通过下调和来治疗2型糖尿病。
结论
本研究表明，在2型糖尿病中通过调节表达诱导β细胞功能障碍，并且它可能是T2DM的一个潜在治疗靶点。
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