Zeng Yuan, Yu Zhengwen, Zhang Yubo, Jiang Chunyan, Huang Xue
School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
School of Agriculture, Anshun University, Anshun 561000, China.
Food Chem X. 2024 Aug 26;23:101783. doi: 10.1016/j.fochx.2024.101783. eCollection 2024 Oct 30.
Exploring natural antioxidants is essential to delay lipid oxidation. This study investigated the inhibitory effect of (AN) extract in six edible oils, compared to TP and TBHQ. Methods included extract preparation, bioactive compounds analysis, in vitro antioxidant activities by FRAP, DPPH, and ABTS assays, fatty acid composition detection, and POV determination. The results showed that AN was rich in total flavonoids, total phenols and had better iron ion reduction ability than TBHQ. In oleic and linoleic acid-rich oils, AN significantly delayed early-stage lipid oxidation, outperforming TP and TBHQ. In linolenic acid-rich oils, AN maintained a stable effect. Molecular docking studies revealed strong binding interactions between main compounds and fatty acids, with Camelliaside A in (7.83) showing higher binding energy to linolenic acid than TBHQ (7.64), supporting the antioxidant effects. These findings suggest AN as a promising natural alternative to synthetic antioxidants, enhancing oil stability and shelf life.
探索天然抗氧化剂对于延缓脂质氧化至关重要。本研究调查了(AN)提取物在六种食用油中的抑制作用,并与TP和TBHQ进行了比较。方法包括提取物制备、生物活性化合物分析、通过FRAP、DPPH和ABTS测定法进行的体外抗氧化活性、脂肪酸组成检测以及POV测定。结果表明,AN富含总黄酮、总酚,并且比TBHQ具有更好的铁离子还原能力。在富含油酸和亚油酸的油中,AN显著延缓了早期脂质氧化,优于TP和TBHQ。在富含亚麻酸的油中,AN保持了稳定的效果。分子对接研究揭示了主要化合物与脂肪酸之间的强结合相互作用,(7.83)中的茶籽糖苷A对亚麻酸的结合能高于TBHQ(7.64),支持了抗氧化作用。这些发现表明AN有望成为合成抗氧化剂的天然替代品,可提高油的稳定性和保质期。