Tea Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Guangzhou 510640, China.
School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Plant Protein Deep Processing, Ministry of Education, South China University of Technology, Guangzhou 510641, China.
Nutrients. 2022 Jul 24;14(15):3037. doi: 10.3390/nu14153037.
Liver injury is a significant public health issue nowadays. Shibi tea is a non- tea prepared from the dried leaves of , one of the plants with the greatest flavonoid concentration, with Camellianin A (CA) being the major flavonoid. Shibi tea is extensively used in food and medicine and has been found to provide a variety of health advantages. The benefits of Shibi tea and CA in preventing liver injury have not yet been investigated. The aim of this study was to investigate the hepatoprotective effects of extract of Shibi tea (EST) and CA in mice with carbon tetrachloride (CCl)-induced acute liver injury. Two different concentrations of EST and CA were given to model mice by gavage for 3 days. Treatment with two concentrations of EST and CA reduced the CCl-induced elevation of the liver index, liver histopathological injury score, alanine aminotransferase (ALT), and aspartate aminotransferase (AST). Western blotting and immunohistochemical analysis demonstrated that EST and CA regulated the oxidative stress signaling pathway protein levels of nuclear factor E2-related factor 2 (Nrf2)/heme-oxygenase-1 (HO-1), the expression of inflammatory cytokines, the phosphorylated nuclear factor-kappaB p65 (p-NF-κB)/nuclear factor-kappaB p65 (NF-κB) ratio, the phospho-p44/42 mitogen-activated protein kinase (p-MAPK), and the apoptosis-related protein levels of BCL2-associated X (Bax)/B cell leukemia/lymphoma 2 (Bcl2) in the liver. Taken together, EST and CA can protect against CCl-induced liver injury by exerting antioxidative stress, anti-inflammation, and anti-apoptosis.
肝损伤是当今一个重大的公共卫生问题。屎毕茶是一种非茶饮品，由 的干叶制成，其中含有最大浓度的类黄酮，主要类黄酮为 Camellianin A (CA)。屎毕茶广泛应用于食品和医药领域，已被发现具有多种健康益处。屎毕茶和 CA 预防肝损伤的作用尚未得到研究。本研究旨在探讨屎毕茶提取物 (EST) 和 CA 对四氯化碳 (CCl) 诱导的急性肝损伤小鼠的保肝作用。通过灌胃给予模型小鼠两种不同浓度的 EST 和 CA，连续 3 天。两种浓度的 EST 和 CA 处理均可降低 CCl 诱导的肝指数、肝组织病理损伤评分、丙氨酸氨基转移酶 (ALT) 和天冬氨酸氨基转移酶 (AST) 的升高。Western blot 和免疫组化分析表明，EST 和 CA 调节了氧化应激信号通路蛋白核因子 E2 相关因子 2 (Nrf2)/血红素加氧酶-1 (HO-1)、炎症细胞因子的表达、磷酸化核因子-κB p65 (p-NF-κB)/核因子-κB p65 (NF-κB) 比值、磷酸化丝裂原激活蛋白激酶 p44/42 (p-MAPK) 和 B 细胞淋巴瘤/白血病-2 (Bcl2)相关 X (Bax) 的凋亡相关蛋白水平。综上所述，EST 和 CA 可通过抗氧化应激、抗炎和抗细胞凋亡来预防 CCl 诱导的肝损伤。
