Naqshbandi Nabat, Tamaddonfard Esmaeal, Erfanparast Amir, Soltanalinejad-Taghiabad Farhad
PhD Candidate, Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2024;15(8):411-416. doi: 10.30466/vrf.2024.2015987.4069. Epub 2024 Aug 15.
Investigating the mechanisms responsible for pain processing of natural and synthetic chemical compounds is necessary to optimize pain management. Curcumin (Cur), the active ingredient of turmeric, exhibits potent analgesic and anti-inflammatory properties by employing multiple mechanisms at the local peripheral, spinal and supra-spinal levels. This study was aimed to investigate the effect of oral administration of Cur on muscle pain induced by intramuscular (IM) injection of formalin. To explore the possible local mechanisms, a cyclooxygenase (COX) inhibitor, diclofenac (Dic) and a COX product, prostaglandin E (PGE), were applied. The IM injection of formalin (25.00 µL, 2.50%) into the gastrocnemius muscle induced two distinct phases of hind leg flinching. A short-lasting (10 min) hind leg lifting was observed following IM injection of PGE (2 µg kg, 25.00 µL). Oral administration of Cur (25.00 and 100 mg kg) and IM injection of 40.00 µg kg Dic attenuated formalin and PGE2 induced nociceptive behaviors. Contra-lateral IM injection of Dic did not change muscle pain induced by ipsilateral IM injection of formalin and PGE. The second phase of formalin induced flinching as well as PGE evoked lifting were more suppressed when 40.00 µg kg Dic and 100 mg kg Cur were used together. Locomotor activity was not changed by the above-mentioned treatments. It was concluded that the reducing effect of muscle pain of Cur might be related to the local inhibition of COX.
研究天然和合成化合物疼痛处理的机制对于优化疼痛管理至关重要。姜黄素（Cur）是姜黄的活性成分，通过在局部外周、脊髓和脊髓上水平采用多种机制，表现出强大的镇痛和抗炎特性。本研究旨在探讨口服Cur对肌肉注射福尔马林诱导的肌肉疼痛的影响。为了探索可能的局部机制，应用了一种环氧化酶（COX）抑制剂双氯芬酸（Dic）和一种COX产物前列腺素E（PGE）。向腓肠肌肌肉注射福尔马林（25.00 μL，2.50%）可诱发后腿退缩的两个不同阶段。肌肉注射PGE（2 μg/kg，25.00 μL）后观察到短暂（10分钟）的后腿抬起。口服Cur（25.00和100 mg/kg）以及肌肉注射40.00 μg/kg Dic可减轻福尔马林和PGE2诱导的伤害性反应行为。对侧肌肉注射Dic并未改变同侧肌肉注射福尔马林和PGE诱导的肌肉疼痛。当使用40.00 μg/kg Dic和100 mg/kg Cur联合使用时，福尔马林诱导的退缩的第二阶段以及PGE诱发的抬起受到更明显的抑制。上述处理并未改变运动活性。得出的结论是，Cur对肌肉疼痛的减轻作用可能与局部抑制COX有关。
