Abdul Lateef Khan Khairunnisa, Sheik Abdul Kader Zainoodin
Advanced Medical & Dental Institute, Universiti Sains Malaysia, Pulau Pinang 13300, Malaysia.
Interdiscip Perspect Infect Dis. 2023 Mar 31;2023:4125588. doi: 10.1155/2023/4125588. eCollection 2023.
Typhoid fever is a multiorgan infectious disease caused by . It is transmitted through fecal oral route and can be fatal without proper treatment. Therefore, early diagnosis of typhoid fever is crucial. In the previous study, we have developed TYPHOIDYNE EIA, which showed excellent synergy between the genus conserved and species-specific antigens in the serodiagnosis of typhoid fever. TYPHOIDYNE EIA can effectively detect and differentiate typhoid patients, typhoid vaccinated subjects, healthy subjects, and subjects with other febrile illnesses. Following the successful development of TYPHOIDYNE EIA, in this report, we further characterize the antigenic components of differentially extracted and spp recognized by IgM, IgG, and IgA antibody isotypes in typhoid patients and possible typhoid carrier by the western blot (WB) assay. The WB characterization revealed a dynamic pattern of recognition, with significant variations in the number of antigenic bands observed between the differentially extracted arrays of antigens. The reactivity of patient's sera was divided into 3 regions, with region 1 (≥55 kDa) showing the strongest reactivity followed by region 2 (54 kDa-34 kDa) and region 3 (<34 kDa). Overall, the good synergy expressed in these bands suggests the potential role of these proteins in differentiating typhoid patients with possible typhoid carrier. The antigenic bands highlighted in this study are also identified as prospective biomarkers for diagnostic use and vaccine development.
伤寒热是一种由……引起的多器官传染病。它通过粪口途径传播，若未经适当治疗可能会致命。因此，伤寒热的早期诊断至关重要。在先前的研究中，我们开发了伤寒热酶免疫测定法（TYPHOIDYNE EIA），该方法在伤寒热的血清诊断中显示出属保守抗原和种特异性抗原之间具有出色的协同作用。TYPHOIDYNE EIA能够有效检测并区分伤寒患者、接种伤寒疫苗的受试者、健康受试者以及患有其他发热性疾病的受试者。在成功开发出TYPHOIDYNE EIA之后，在本报告中，我们通过蛋白质印迹（WB）分析进一步表征了在伤寒患者和可能的伤寒携带者中被IgM、IgG和IgA抗体亚型识别的差异提取的……和……属细菌的抗原成分。WB表征揭示了一种动态的识别模式，在差异提取的抗原阵列之间观察到的抗原条带数量存在显著差异。患者血清的反应性分为3个区域，区域1（≥55 kDa）显示出最强的反应性，其次是区域2（54 kDa - 34 kDa）和区域3（<34 kDa）。总体而言，这些条带中表现出的良好协同作用表明这些蛋白质在区分伤寒患者和可能的伤寒携带者方面具有潜在作用。本研究中突出显示的抗原条带也被确定为用于诊断和疫苗开发的潜在生物标志物。
