Corum Orhan, Turk Erdinc, Durna Corum Duygu, Terzi Ertugrul, Cellat Mustafa, Yıldırım Önder, Uney Kamil
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal, Hatay, Türkiye.
Department of Veterinary Medicine, Devrekani TOBB Vocational School, University of Kastamonu, Kastamonu, Türkiye.
PeerJ. 2024 Sep 11;12:e17973. doi: 10.7717/peerj.17973. eCollection 2024.
The aim of this study was to determine the plasma pharmacokinetics of oxytetracycline (OTC) in rainbow trout () of different body sizes.
The research was carried out on three groups as small (30-50 g), medium (90-110 g) and large (185-215 g) body sizes at 8 ± 0.5 °C. OTC was administered orally at a dose of 60 mg/kg to all groups. Blood samples were taken at 19 different sampling times until the 384 h after oxytetracycline administration. The plasma concentrations of OTC were measured using high pressure liquid chromatography-ultraviolet and pharmacokinetic parameters were evaluated using non-compartmental analysis.
OTC was detected in small-body sized fish until the 336 h and in medium and large-body sized fish until the 384 h. The elimination half-life of OTC was 85.46, 87.24 and 86.98 h in the small, medium and large body size groups, respectively. The peak plasma concentration increased from 0.66 to 1.11 µg/mL, and the area under the plasma concentration-versus time curve from zero (0) h to infinity (∞) increased from 87.86 to 151.52 h*µg/mL, in tandem with the increase in fish body size. As fish body size increased, volume of distribution and total body clearance decreased.
These results show that the pharmacokinetics of OTC vary depending on fish size. Therefore, there is a need to reveal the pharmacodynamic activity of OTC in rainbow trout of different body sizes.
本研究旨在确定不同体型虹鳟鱼体内土霉素（OTC）的血浆药代动力学。
研究在8±0.5℃下对三组不同体型的虹鳟鱼进行，分别为小体型（30 - 50克）、中体型（90 - 110克）和大体型（185 - 215克）。所有组均以60毫克/千克的剂量口服给予OTC。在土霉素给药后384小时内的19个不同采样时间采集血样。使用高压液相色谱 - 紫外法测定血浆中OTC的浓度，并使用非房室分析评估药代动力学参数。
在小体型鱼中直到336小时检测到OTC，在中体型和大体型鱼中直到384小时检测到OTC。小、中、大体型组中OTC的消除半衰期分别为85.46、87.24和86.98小时。随着鱼体大小的增加，血浆峰浓度从0.66微克/毫升增加到1.11微克/毫升，血浆浓度 - 时间曲线下从零（0）小时到无穷大（∞）的面积从87.86小时微克/毫升增加到151.52小时微克/毫升。随着鱼体大小增加，分布容积和全身清除率降低。
这些结果表明OTC的药代动力学因鱼的大小而异。因此，有必要揭示OTC在不同体型虹鳟鱼中的药效学活性。
