Ajakkala Pallavi Bhat, Nayak Srajana, Maiti Biswajit, Rohit Anusha, Mohan Raj Juliet Roshini, Karunasagar Indrani
NITTE (Deemed to Be University), NITTE University Centre for Science Education and Research, Mangaluru, 575018 Karnataka India.
Department of Microbiology, Madras Medical Mission, Chennai, 600037 Tamil Nadu India.
Indian J Microbiol. 2024 Sep;64(3):1379-1383. doi: 10.1007/s12088-024-01217-6. Epub 2024 Jun 5.
Multidrug-resistant (MDR-KP) infections have become a major global issue in the healthcare sector. Alternative viable tactics for combating bacterial infections, such as the use of bacteriophages, can be considered. One of the major challenges in phage therapy is the emergence of phage-resistant bacteria. This study isolated bacteriophages from water and soil samples against MDR-KP isolates. Susceptible bacterial hosts were exposed to phages at different concentrations and prolonged durations of time to obtain phage-resistant survivors. Phenotypic changes such as changes in growth rates, biofilm formation ability, antibiotic sensitivity patterns, and outer membrane proteins (OMPs) profiling of the survivors were studied. Our findings indicate that the phage ØKp11 and ØKp26 survivors had reduced growth rates and biofilm formation ability, altered antibiotic sensitivity patterns, and reduced OMPs expression compared with the parent MDR-KP002 isolate. These results suggest that the alternations in the bacterial envelope result in phenotypic phage resistance among MDR bacterial isolates.
The online version contains supplementary material available at 10.1007/s12088-024-01217-6.
耐多药肺炎克雷伯菌（MDR-KP）感染已成为医疗保健领域的一个重大全球问题。可以考虑采用其他可行的策略来对抗细菌感染，例如使用噬菌体。噬菌体疗法的主要挑战之一是噬菌体抗性细菌的出现。本研究从水和土壤样本中分离出针对MDR-KP分离株的噬菌体。将敏感细菌宿主暴露于不同浓度和延长时间的噬菌体中，以获得噬菌体抗性存活菌。研究了存活菌的表型变化，如生长速率变化、生物膜形成能力、抗生素敏感性模式以及外膜蛋白（OMPs）谱。我们的研究结果表明，与亲本MDR-KP002分离株相比，噬菌体ØKp11和ØKp26的存活菌生长速率降低、生物膜形成能力降低、抗生素敏感性模式改变且OMPs表达减少。这些结果表明，细菌包膜的改变导致MDR细菌分离株产生表型噬菌体抗性。
在线版本包含可在10.1007/s12088-024-01217-6获取的补充材料。
