Gil-Gil Teresa, Berryhill Brandon A
Department of Biology, Emory University; Atlanta, Georgia, 30322, USA.
Program in Microbiology and Molecular Genetics, Graduate Division of Biological and Biomedical Sciences, Laney Graduate School, Emory University; Atlanta, GA, 30322, USA.
bioRxiv. 2024 Sep 6:2024.09.06.611640. doi: 10.1101/2024.09.06.611640.
There is a long-standing belief that bacteriostatic drugs are inherently antagonistic to the action of bactericidal antibiotics. This belief is primarily due to the fact that the action of most bactericidal antibiotics requires the target bacteria to be growing. Since bacteriostatic drugs stop the growth of treated bacteria, these drugs would necessarily work against one another. We have recently shown that bacteria treated with high concentrations of bacteriostatic drugs retain some metabolic activity, dividing on average once per day.
We seek to determine if this low level of growth is sufficient to allow for bactericidal antibiotics of different classes to still kill after bacteria are treated with bacteriostatic drugs.
We first treated and with two different bacteriostatic drugs, followed by one of three bactericidal drugs of three different classes. The density of these bacteria was tracked over six days to determine the amount of killing that occurred.
Our results question this long-standing belief by demonstrating conditions where sequential treatment with a bacteriostatic then bactericidal antibiotic is as or more effective than treatment with a bactericidal drug alone.
These results raise the need to investigate the pharmacodynamics of the joint action of bacteriostatic and bactericidal antibiotics and .
长期以来，人们一直认为抑菌药物本质上与杀菌抗生素的作用相互拮抗。这种观点主要源于大多数杀菌抗生素的作用需要靶细菌处于生长状态这一事实。由于抑菌药物会阻止被治疗细菌的生长，所以这些药物必然会相互拮抗。我们最近发现，用高浓度抑菌药物处理过的细菌仍保留一些代谢活性，平均每天分裂一次。
我们试图确定这种低水平的生长是否足以使不同类别的杀菌抗生素在细菌经抑菌药物处理后仍能发挥杀菌作用。
我们先用两种不同的抑菌药物处理[具体细菌名称未给出]和[具体细菌名称未给出]，然后再用三种不同类别的三种杀菌药物中的一种进行处理。在六天内跟踪这些细菌的密度，以确定发生的杀菌量。
我们的结果对这一长期存在的观点提出了质疑，结果表明，先使用抑菌药物然后再使用杀菌抗生素的序贯治疗，与单独使用杀菌药物相比，效果相同或更好。
这些结果表明有必要研究抑菌抗生素和杀菌抗生素联合作用的药效学。
