Yemma J J, Perry L A
Cytobios. 1985;43(171):115-29.
Quantitative cytochemical determinations were made of the DNA of zygotes formed from myxamoebae and swarm cells of Didymium iridis. The nuclear and cytoplasmic RNA and lysine bound protein of these cells were also measured. Significant zygote formation in myxamoebae crosses began at 20-30 min, while swarmers required 35-40 min. Myxamoebae, however, demonstrated a greater ability to form zygotes. The total cytoplasmic RNA and protein bound lysine for myxamoebae was higher than that of the swarmer cells. This observed decrease in swarmers may be due to reduced protein synthesis. Values for nuclear RNA were higher in the myxamoebae, but nuclear lysine bound protein was higher in the swarmers. The data presented suggest that prefusion swarmers, after replicating their DNA, go into a period of G2 arrest and remain in this condition postfusion. In contrast, prefusion myxamoebae readily divide after DNA replication, and continue to synthesize nuclear DNA, and to divide after fusion.
对双滴绒泡菌的变形体和游动细胞形成的合子的DNA进行了定量细胞化学测定。还测量了这些细胞的细胞核和细胞质RNA以及赖氨酸结合蛋白。变形体杂交中显著的合子期形成始于20-30分钟，而游动细胞需要35-40分钟。然而，变形体表现出了更强的形成合子的能力。变形体的总细胞质RNA和结合赖氨酸的蛋白高于游动细胞。观察到的游动细胞中的这种减少可能是由于蛋白质合成减少。变形体的细胞核RNA值较高，但游动细胞中细胞核赖氨酸结合蛋白较高。所呈现的数据表明，融合前的游动细胞在复制其DNA后进入G2期停滞，并在融合后保持这种状态。相比之下，融合前的变形体在DNA复制后很容易分裂，并继续合成细胞核DNA，并在融合后继续分裂。
