胰腺A细胞中胰高血糖素分泌与细胞内钙分布
Glucagon secretion and intracellular calcium distribution in pancreatic A cells.
作者信息
Rebolledo O R, Semino C, Gagliardino J J
出版信息
Endocrinology. 1985 Oct;117(4):1707-9. doi: 10.1210/endo-117-4-1707.
Quantitative changes in the distribution of intracellular calcium in A cells from perfused rat pancreas in relation to the secretory state of A cells were studied with the pyroantimonate technique for calcium precipitation. A cells stimulated with a 3.3 mM glucose concentration in the perfusate presented numerous calcium precipitates attached to cell membranes, nucleus, cytoplasm and secretion granules. Independently of the length of treatment, inhibition of glucagon release with 16.6 mM glucose decreased the calcium precipitates in every cell organelle studied. These results suggest that intracellular calcium rearrangement might be important in coupling stimulus to secretion in A cells, as it has been demonstrated for pancreatic B cells.
采用焦锑酸盐技术沉淀钙，研究了灌流大鼠胰腺A细胞内钙分布的定量变化与A细胞分泌状态的关系。用含3.3 mM葡萄糖的灌流液刺激A细胞时，可见大量钙沉淀附着于细胞膜、细胞核、细胞质和分泌颗粒上。无论处理时间长短，用16.6 mM葡萄糖抑制胰高血糖素释放都会减少所研究的每个细胞器中的钙沉淀。这些结果表明，细胞内钙重排可能在A细胞的刺激与分泌偶联中起重要作用，正如在胰腺B细胞中所证实的那样。
Zotero 插件