Health and Disease Omics Center, Chiba University, Chiba, Japan.
Department of Molecular Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan.
Methods Mol Biol. 2025;2856:3-9. doi: 10.1007/978-1-0716-4136-1_1.
Recent analyses revealed the essential function of chromatin structure in maintaining and regulating genomic information. Advancements in microscopy, nuclear structure observation techniques, and the development of methods utilizing next-generation sequencers (NGSs) have significantly progressed these discoveries. Methods utilizing NGS enable genome-wide analysis, which is challenging with microscopy, and have elucidated concepts of important chromatin structures such as a loop structure, a domain structure called topologically associating domains (TADs), and compartments. In this chapter, I introduce chromatin interaction techniques using NGS and outline the principles and features of each method.
最近的分析揭示了染色质结构在维持和调节基因组信息方面的重要功能。显微镜技术、核结构观察技术的进步以及利用下一代测序仪（NGS）的方法的发展，极大地推动了这些发现。利用 NGS 的方法可以进行全基因组分析，这是显微镜技术所难以实现的，并且阐明了重要的染色质结构的概念，如环结构、拓扑关联结构域（TAD）等结构域和隔室。在本章中，我将介绍利用 NGS 的染色质相互作用技术，并概述每种方法的原理和特点。
