Tilney N L, Garovoy M R, Busch G J, Strom T B, Graves M J, Carpenter C B
Transplantation. 1979 Nov;28(5):421-6. doi: 10.1097/00007890-197911000-00015.
Viable infiltrating host leukocytes have been isolated from 10 rejected human renal allografts, removed 1 to 67 months after transplantation. The cell populations have been identified by surface characteristics and their cytotoxic capacities were assessed. A heterogenous population of cells of host origin accumulated in the grafts, including T and B lymphocytes, Fc+ cells, and macrophages. Using a 51Cr release assay, specific cytotoxicity against donor alloantigens was determined. Cytotoxicity of the infiltrating cells was almost invariably greater than cytotoxicity mounted by recipient peripheral blood lymphocytes. Deletion studies confirmed previous work and suggested that T cells were primarily responsible for cytolysis in early acute rejection; non-T cells more often in late chronic rejection. Antibodies eluted from the grafts demonstrated both specific antidonor and nonspecific activity as well as cross-reacting anti-HLA activity. Allograft morphology was examined and cellular and humoral host responses were assessed. These studies emphasize the complexities of immune responses produced by the host against transplanted tissues.
已从10例移植后1至67个月被排斥的人肾移植受者中分离出存活的浸润性宿主白细胞。通过表面特征鉴定细胞群体，并评估其细胞毒性能力。移植器官中积累了宿主来源的异质性细胞群体，包括T淋巴细胞、B淋巴细胞、Fc +细胞和巨噬细胞。使用51Cr释放试验测定针对供体同种异体抗原的特异性细胞毒性。浸润细胞的细胞毒性几乎总是大于受体外周血淋巴细胞产生的细胞毒性。缺失研究证实了先前的工作，并表明T细胞在早期急性排斥反应中主要负责细胞溶解；在晚期慢性排斥反应中，非T细胞更常见。从移植器官中洗脱的抗体表现出特异性抗供体和非特异性活性以及交叉反应性抗HLA活性。检查了同种异体移植的形态，并评估了细胞和体液宿主反应。这些研究强调了宿主对移植组织产生的免疫反应的复杂性。
