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从供应尿素的硝化群落中分离出一株中度嗜酸硝化杆菌。

Isolation of a Moderately Acidophilic Nitrobacter from a Nitrifying Community Supplied with Urea.

作者信息

Endo Yuta, Fujitani Hirotsugu, Kaneko Ayano, Ninomiya Takuya, Umezawa Chiharu, Kuroiwa Megumi, Suwa Yuichi

机构信息

Department of Biological Sciences, Chuo University.

出版信息

Microbes Environ. 2024;39(3). doi: 10.1264/jsme2.ME24027.

DOI:10.1264/jsme2.ME24027
PMID:39284717
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11427310/
Abstract

Nitrite-oxidizing bacteria (NOB), which perform the second step of aerobic nitrification, play an important role in soil. In the present study, we report a novel isolate from agricultural soil affiliated with the genus Nitrobacter and its physiological characteristics. We sampled the surface soil of a vegetable field and obtained mixed culture A31 using the most probable number (MPN) method with inorganic medium containing 0.75‍ ‍mM urea (pH 5.5). The dilution-extinction procedure on culture A31 led to the isolation of a strain that was designated as Nitrobacter sp. A67. The nxrB1 gene sequence of Nitrobacter sp. A67 (302 bp) was classified into Cluster 5, and the highest sequence identity was 96.10% with Nitrobacter sp. BS5/19. The NO oxidation activity of Nitrobacter sp. A67 was investigated at various pH. The optimum pH for NO oxidation was 5.8-6.4. This result indicates that Nitrobacter sp. A67 is a moderately acidophilic nitrite-oxidizing bacterium.

摘要

亚硝酸氧化细菌（NOB）在土壤中发挥着重要作用，它们进行需氧硝化作用的第二步。在本研究中，我们报告了一种从农业土壤中分离出的与硝化杆菌属相关的新型菌株及其生理特性。我们采集了一块菜地的表层土壤，并使用含有0.75 mM尿素（pH 5.5）的无机培养基，通过最大可能数（MPN）法获得了混合培养物A31。对培养物A31进行稀释-灭绝程序后，分离出了一株被命名为硝化杆菌属A67的菌株。硝化杆菌属A67的nxrB1基因序列（302 bp）被归类为第5簇，与硝化杆菌属BS5/19的最高序列同一性为96.10%。我们研究了硝化杆菌属A67在不同pH值下的NO氧化活性。NO氧化的最适pH值为5.8 - 6.4。这一结果表明硝化杆菌属A67是一种中度嗜酸的亚硝酸氧化细菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/7439da6d592a/39_24027-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/0139b7c124aa/39_24027-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/542f6c1717dc/39_24027-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/bc0b55f45fc3/39_24027-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/9fabdd33c69e/39_24027-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/7439da6d592a/39_24027-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/0139b7c124aa/39_24027-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/542f6c1717dc/39_24027-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/bc0b55f45fc3/39_24027-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/9fabdd33c69e/39_24027-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ecb/11427310/7439da6d592a/39_24027-g005.jpg

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