Ma Guangyu, Li Yimi, Tye Kian Deng, Huang Ting, Tang Xiaomei, Luo Huijuan, Wang Dongju, Zhou Juan, Li Zhe, Xiao Xiaomin
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Department of Obstetrics and Gynecology, Dangyang People's Hospital, Dangyang, China.
Heliyon. 2024 Aug 30;10(17):e37157. doi: 10.1016/j.heliyon.2024.e37157. eCollection 2024 Sep 15.
The main aim of this study was to evaluate the effect of oral probiotics on the human milk microbiota and determine whether that influenced infant microbiota development.
A total of 27 pregnant women were recruited; 14 were assigned to the probiotic group, and the rest were assigned to the control group. Their infants were likewise assigned to the probiotic group or the control group. Pregnant women in the probiotic group received probiotic supplementation from 32 weeks of gestation until delivery. Human milk samples and infant fecal samples were collected at 6 months after delivery, and 16S rRNA sequencing was used to analyze the composition of the human milk and infant gut microbiota (NCT06241222).
In the control group, bacterial microbiota were detected in 8 out of 13 milk samples, whereas in the probiotic group, only 6 out of 14 milk samples contained bacterial microbiota. We examined the composition of the human milk and infant gut microbiota in both the control and probiotic groups. Spearman correlation analysis revealed that various genera in human milk were correlated with the infant gut microbiota. The Linear discriminant analysis effect size (LEfSe) showed that 6 bacteria in the human milk microbiota in the control group were significantly more abundant than those in the probiotic group. Nine bacteria were significantly more abundant in the human milk microbiota in the probiotic group than the control group. According to the LEfSe results, 11 bacteria in the infant gut microbiota in the control group were significantly more abundant than those in the probiotic group. Fourteen bacteria were significantly more abundant in the infant gut microbiota in the probiotic group than in the control group.
The infant gut microbiota at 6 months has a complicated relationship with the maternal human milk microbiota. Oral probiotic supplementation can change the composition of the human milk microbiota and the infant gut microbiota.
本研究的主要目的是评估口服益生菌对人乳微生物群的影响，并确定这是否会影响婴儿微生物群的发育。
共招募了27名孕妇；14名被分配到益生菌组，其余被分配到对照组。她们的婴儿同样被分配到益生菌组或对照组。益生菌组的孕妇从妊娠32周开始直至分娩接受益生菌补充。在分娩后6个月收集人乳样本和婴儿粪便样本，并使用16S rRNA测序分析人乳和婴儿肠道微生物群的组成（NCT06241222）。
在对照组中，13份乳汁样本中有8份检测到细菌微生物群，而在益生菌组中，14份乳汁样本中只有6份含有细菌微生物群。我们检查了对照组和益生菌组中人乳和婴儿肠道微生物群的组成。Spearman相关性分析显示，人乳中的各种菌属与婴儿肠道微生物群相关。线性判别分析效应大小（LEfSe）表明，对照组人乳微生物群中的6种细菌比益生菌组中的明显更丰富。益生菌组人乳微生物群中的9种细菌比对照组中的明显更丰富。根据LEfSe结果，对照组婴儿肠道微生物群中的11种细菌比益生菌组中的明显更丰富。益生菌组婴儿肠道微生物群中的14种细菌比对照组中的明显更丰富。
6个月时的婴儿肠道微生物群与母体人乳微生物群有着复杂的关系。口服益生菌补充剂可以改变人乳微生物群和婴儿肠道微生物群的组成。
