N. N. Petrov National Medical Cancer Research Center, Ministry of Health of the Russian Federation, St. Petersburg, Russia.
Bull Exp Biol Med. 2024 Aug;177(4):564-568. doi: 10.1007/s10517-024-06226-5. Epub 2024 Sep 17.
Homologous animal cell product was obtained in protocol developed for female BALB/c mice. Dendritic cell (DC) migration from the injection site into the draining lymph nodes was evaluated. The number of DC labeled with carboxyfluorescein succinimidyl ester (CFSE) in draining lymph nodes increased from 5.3% (16 h) to 13.3% (48 h) (p=0.028) with a maximum at 72 h (15.4%, p=0.003). The immunophenotype of CFSE-DC detected in murine lymph nodes corresponded to the immunophenotype of mature vaccine DCs: they expressed differentiation markers CD11c, CD80, CD83, and CD86 (p>0.05 vs initial DC).
方案中获得了同源动物细胞产物。评估了树突状细胞（DC）从注射部位迁移到引流淋巴结的情况。用羧基荧光素琥珀酰亚胺酯（CFSE）标记的引流淋巴结中的 DC 数量从 5.3%（16 小时）增加到 13.3%（48 小时）（p=0.028），72 小时时达到最大值（15.4%，p=0.003）。在鼠类淋巴结中检测到的 CFSE-DC 的免疫表型与成熟疫苗 DC 的免疫表型相对应：它们表达分化标志物 CD11c、CD80、CD83 和 CD86（p＞0.05 与初始 DC 相比）。
