OsNF-YB7 使 OsGLK1 失活，从而抑制水稻胚中的叶绿素合成。

OsNF-YB7 inactivates OsGLK1 to inhibit chlorophyll biosynthesis in rice embryo.

机构信息

Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/ Zhongshan Biological Breeding Laboratory, Agricultural College of Yangzhou University, Yangzhou, China.

Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/ Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou, China.

出版信息

Elife. 2024 Sep 17;13:RP96553. doi: 10.7554/eLife.96553.

DOI:10.7554/eLife.96553

PMID:39288070

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11407766/

Abstract

As a master regulator of seed development, Leafy Cotyledon 1 (LEC1) promotes chlorophyll (Chl) biosynthesis in , but the mechanism underlying this remains poorly understood. Here, we found that loss of function of , a homolog of rice, leads to chlorophyllous embryo, indicating that plays an opposite role in Chl biosynthesis in rice compared with that in . OsNF-YB7 regulates the expression of a group of genes responsible for Chl biosynthesis and photosynthesis by directly binding to their promoters. In addition, OsNF-YB7 interacts with Golden 2-Like 1 (OsGLK1) to inhibit the transactivation activity of OsGLK1, a key regulator of Chl biosynthesis. Moreover, OsNF-YB7 can directly repress expression by recognizing its promoter in vivo, indicating the involvement of OsNF-YB7 in multiple regulatory layers of Chl biosynthesis in rice embryo. We propose that OsNF-YB7 functions as a transcriptional repressor to regulate Chl biosynthesis in rice embryo.

摘要

作为种子发育的主要调节因子，LEAFY COTYLEDON1（LEC1）促进中叶绿素（Chl）的生物合成，但这一机制尚不清楚。在这里，我们发现水稻的同源物的功能丧失会导致叶色胚胎，表明在水稻的 Chl 生物合成中与的作用相反。OsNF-YB7 通过直接结合其启动子来调节一组负责 Chl 生物合成和光合作用的基因的表达。此外，OsNF-YB7 与 Golden 2-Like 1（OsGLK1）相互作用，抑制 Chl 生物合成的关键调节因子 OsGLK1 的转录激活活性。此外，OsNF-YB7 可以通过在体内识别其启动子来直接抑制的表达，表明 OsNF-YB7 参与了水稻胚胎中 Chl 生物合成的多个调控层。我们提出，OsNF-YB7 作为一种转录抑制剂在水稻胚胎中发挥作用，调节 Chl 生物合成。