Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, China.
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, China.
Talanta. 2025 Jan 1;281:126888. doi: 10.1016/j.talanta.2024.126888. Epub 2024 Sep 14.
Serotonin is an essential neurotransmitter that regulates many physiological processes and is related to a variety of diseases. Herein, a novel electrochemiluminescence-resonance energy transfer (ECL-RET) aptasensor for serotonin detection was developed, with zinc-based metal-organic frameworks (Zn-MOFs) as an ECL donor and Pt@CuO cubic nanocrystals (CNs) as an acceptor. In the presence of target, numerous Pt@CuO CNs were brought to electrode surface through the catalytic hairpin assembly (CHA)-driven DNA walker, resulting in a significant inhibition of ECL signal. The efficient ECL-RET device exhibited a wide linear range for monitoring serotonin (10 to 10 M) and a low detection limit of 0.5 pM. Furthermore, satisfactory recoveries were obtained by using the aptasensor to monitor serotonin levels in serum and urine samples. The broadband absorption feature of Pt@CuO CNs, along with the extraordinary amplification effect of catalytic hairpin assembly (CHA)-driven DNA walking machine, provided a new route for the construction of efficient ECL-RET systems.
血清素是一种重要的神经递质，调节许多生理过程，与多种疾病有关。在此，开发了一种新型的电化学发光共振能量转移（ECL-RET）适体传感器用于检测血清素，其中锌基金属-有机框架（Zn-MOFs）作为 ECL 供体，Pt@CuO 立方纳米晶体（CNs）作为受体。在存在靶标的情况下，通过催化发夹组装（CHA）驱动的 DNA walker 将大量的 Pt@CuO CNs 带到电极表面，导致 ECL 信号显著抑制。该高效的 ECL-RET 器件在监测血清素（10 到 10 M）时表现出宽线性范围和 0.5 pM 的低检测限。此外，该适体传感器在监测血清和尿液样本中的血清素水平时获得了令人满意的回收率。Pt@CuO CNs 的宽带吸收特性以及催化发夹组装（CHA）驱动的 DNA 行走机器的非凡放大效应，为构建高效 ECL-RET 系统提供了新途径。
