Fleischmann J, Heston W D, Bander N H, Bauer W C, Janney C, Ratliff T L, Fair W R
J Urol. 1985 Sep;134(3):570-4. doi: 10.1016/s0022-5347(17)47302-2.
A primary human renal clear cell carcinoma has been developed as a xenograft (JDF-1). Passage 7 of the JDF-1 tumor retained the microscopic morphology of the primary tumor, electron micrographs have confirmed its epithelial characteristics and karyotyping of a subsequent passage has proved it is not a murine hybrid. Immunoperoxidase studies using a panel of murine monoclonal antibodies demonstrate an antigenic phenotype specific for human renal cancer. In vitro chemosensitivities of the JDF-1 tumor were determined by the double-layer soft-agar clonogenic assay method. JDF-1 showed no significant sensitivities to several standard chemotherapeutic agents, but alpha-2-interferon and difluoromethylornithine in combination synergistically inhibited its growth by 74 per cent.
一种原发性人类肾透明细胞癌已被开发成异种移植物（JDF-1）。JDF-1肿瘤的第7代保留了原发性肿瘤的微观形态，电子显微镜照片证实了其上皮特征，后续传代的核型分析证明它不是鼠类杂交瘤。使用一组鼠单克隆抗体的免疫过氧化物酶研究显示了对人类肾癌具有特异性的抗原表型。JDF-1肿瘤的体外化学敏感性通过双层软琼脂克隆形成试验方法来确定。JDF-1对几种标准化疗药物没有明显敏感性，但α-2-干扰素和二氟甲基鸟氨酸联合使用可协同抑制其生长达74%。
