Department of Food Engineering, Faculty of Engineering, Aksaray University, 68100, Aksaray, Turkey.
Department of Food Engineering, Faculty of Engineering, Bolu Abant Izzet Baysal University, 14030, Bolu, Turkey.
Curr Microbiol. 2024 Sep 17;81(11):364. doi: 10.1007/s00284-024-03881-9.
This study evaluated the in vitro probiotic potential and postbiotic properties of yeast strains isolated from traditional fermented foods, emphasizing antioxidant activity (AOA) and biofilm inhibition capacity (BIC). The yeasts were molecularly confirmed using start codon targeted polymorphisms as Kluyveromyces lactis (n = 17), Saccharomyces cerevisiae (n = 9), Pichia kudriavzevii (n = 6), P. fermentans (n = 4), Wickerhamomyces anomalus (n = 2), and Torulaspora delbrueckii (n = 1). The probiotic assessment of live cells included viability in simulated gastric and pancreatic juices, autoaggregation, hydrophobicity, and AOA, using S. boulardii MYA-796 as reference. Additionally, cell-free supernatants (CFS) were tested for AOA and BIC against Cronobacter sakazakii, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus. Several strains exhibited significantly higher in vitro probiotic characteristics compared to S. boulardii MYA-796 (P < 0.05), particularly in gastric and pancreatic survival, hydrophobicity, and AOA. Notably, CFS exhibited greater AOA than live cells and strong BIC, especially against L. monocytogenes and S. aureus. Multivariate analysis identified K. lactis TC11, S. cerevisiae M33T1-2, P. kudriavzevii S96, W. anomalus OB7Y1, and T. delbrueckii KY31 as having superior probiotic properties, attributed to enhanced gastric survival, autoaggregation, and AOA. CFS of S. cerevisiae M33T1-2 and T. delbrueckii KY31 demonstrated significant BIC, with over 60% inhibition across all tested pathogens.
本研究评估了从传统发酵食品中分离出的酵母菌株的体外益生菌潜力和后生元特性，重点是抗氧化活性（AOA）和生物膜抑制能力（BIC）。使用起始密码子靶向多态性对酵母进行了分子鉴定，鉴定结果为：乳克鲁维酵母（n = 17）、酿酒酵母（n = 9）、毕赤酵母（n = 6）、发酵毕赤酵母（n = 4）、异常汉逊酵母（n = 2）和德巴利汉逊酵母（n = 1）。活细胞的益生菌评估包括在模拟胃液和胰液中的生存能力、自聚集、疏水性和 AOA，以 S. boulardii MYA-796 作为参考。此外，还测试了无细胞上清液（CFS）的 AOA 和针对克罗诺杆菌、单核细胞增生李斯特菌、铜绿假单胞菌和金黄色葡萄球菌的 BIC。与 S. boulardii MYA-796 相比，一些菌株表现出显著更高的体外益生菌特性（P < 0.05），特别是在胃和胰腺的生存能力、疏水性和 AOA 方面。值得注意的是，CFS 表现出比活细胞更强的 AOA 和强大的 BIC，特别是针对单核细胞增生李斯特菌和金黄色葡萄球菌。多元分析确定 K. lactis TC11、S. cerevisiae M33T1-2、P. kudriavzevii S96、W. anomalus OB7Y1 和 T. delbrueckii KY31 具有优越的益生菌特性，归因于增强的胃生存能力、自聚集和 AOA。S. cerevisiae M33T1-2 和 T. delbrueckii KY31 的 CFS 表现出显著的 BIC，对所有测试病原体的抑制率超过 60%。
